ID   SYGB_XANCB              Reviewed;         698 AA.
AC   B0RYX3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=xcc-b100_4289;
OS   Xanthomonas campestris pv. campestris (strain B100).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=509169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B100;
RX   PubMed=18304669; DOI=10.1016/j.jbiotec.2007.12.013;
RA   Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T.,
RA   Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K.,
RA   Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K.,
RA   Puehler A.;
RT   "The genome of Xanthomonas campestris pv. campestris B100 and its use for
RT   the reconstruction of metabolic pathways involved in xanthan
RT   biosynthesis.";
RL   J. Biotechnol. 134:33-45(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AM920689; CAP53659.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0RYX3; -.
DR   SMR; B0RYX3; -.
DR   KEGG; xca:xcc-b100_4289; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000001188; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..698
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101371"
SQ   SEQUENCE   698 AA;  75049 MW;  3DF69C48AB136303 CRC64;
     MSEQLPLLIE LGTEELPVKA LPGLAQAFFD GVLAGLEKRG VAVTRGDAKP LSTPRRLAVL
     LPGVATEQPE QRSEVLGPYL NIALDAEGKP TRALAGFAAK AGIDWTALER TSDAKGERFV
     HRAVTPGAQA AALLPEILRE AIAAMPIPKP MRWGAHEYAF ARPVQWLVLL FGDTVIPAEL
     LGVRGDRITR GHRFMHDGDI ALAAPGDYID ALRAAHVLVD ADARRARIVE EVDAAARQAG
     GSARISDDNL EQVVNLVEWP SAVLCSFERA FLAVPQEALI ETMEINQKFF PVLDDGGKLT
     EQFIGIANIV SKDVAEVAKG YERVIRPRFA DAKFFFDEDL KQGLEAMGAG LASVTYQAKL
     GTVADKVARV AALAEAIAPQ VGADPVQARR AAELAKNDLQ SRMVNEFPEL QGIAGRHYAK
     AAGEPSEISL AIDEAYQPRF AGDDIALSPL GKVLAIAERL DTLAGGFAAG LKPTGNKDPF
     ALRRNALGLA RTVIESGFDL DLPKLIDVGL ASLPDAVKPH ADRNTETVRA DLYDFILDRL
     KGYYADKGVA ATHFNAVAEL TPASLYDFDR RIDAIGIFAT LPEAEALAAA NKRIRNILRK
     VEGEIPGDID TTLLREPAEE ALAEAVEAAI GDTGDALHRH DYVAVLARLA RLRPQVDAFF
     DGVMVNADDP QLRANRLALL KKLGDRLGSV AAIEHLSS