ID   SYGB_XYLFM              Reviewed;         722 AA.
AC   B0U7B2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Xfasm12_1013;
OS   Xylella fastidiosa (strain M12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xylella.
OX   NCBI_TaxID=405440;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M12;
RX   PubMed=20601474; DOI=10.1128/jb.00651-10;
RA   Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L.;
RT   "Whole genome sequences of two Xylella fastidiosa strains (M12 and M23)
RT   causing almond leaf scorch disease in California.";
RL   J. Bacteriol. 192:4534-4534(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000941; ACA11978.1; -; Genomic_DNA.
DR   RefSeq; WP_004085462.1; NC_010513.1.
DR   AlphaFoldDB; B0U7B2; -.
DR   SMR; B0U7B2; -.
DR   PRIDE; B0U7B2; -.
DR   KEGG; xfm:Xfasm12_1013; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..722
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101373"
SQ   SEQUENCE   722 AA;  80181 MW;  93801C029356A52D CRC64;
     MTELKPLLIE LGTEELPVNA LPSLSRAFFE GVLAGLERRG IVIDHGEAKS LSTPRRLAVL
     LTAVAVEQPK QYRELFGPYL NTAFDTEGKP TKALEGFAAK CGVNWRTLER IRDTKGERFV
     HRVVVPGERT DALLPGILTE AIAGMPIPKP MRWGAHEYLF ARPVHWLVML FGQDVVEAEI
     MGVKAGRISR GHRFLYDKPV SLSDPQNYID VLQAAYVLVD PAARRARIVA EIEAVARQVG
     GVARITEDNV AQVVNLVEWP SAVLCSFERV FLEVPQEALI QTMEVNQKFF PVLDSLGKLT
     EKFIGIANIE SNDVAEVAKG YERVIRPRFS DAKFFFNEDL KQGLKAMGER LRTVTYHAKL
     GTLADKVARV LVLAEAIAPQ IGVDPLLARR VALLSKNDLQ SRMVNEFPEL QGVAGHHYAL
     ISGELPEVAM AIEDAYRPRF SGDEIARSPL GKVLGLAERL DTLACGFAVG MKPTGNKDPF
     GLRRNALGLA RTIIESRFDL DLKVLLDQAS DWVAFATTIE QERHAQESVK QSKKEAAVKH
     SLPQVSDEKS ARIEELYDFI VERLRGYYAD KGIPTTHFNA VAELKPVSLY DFHRRLDAIG
     RFAALPEAEA LAVANKRIRN ILRKAEIKIP ASVDATLFDQ PAESGLLVAL EGVITDTRSA
     LDCKNYVSVL TCLARLRPPI DEFFDKVMVN DENLMLRANR LALLQRLGEY LCCVAAIEHL
     SN