SYG_CHLPN
ID SYG_CHLPN Reviewed; 1010 AA.
AC Q9Z6W0; Q9JS87; Q9K1V6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 25-MAY-2022, entry version 144.
DE RecName: Full=Glycine--tRNA ligase;
DE AltName: Full=Glycyl-tRNA synthetase;
DE Short=GlyRS;
DE EC=6.1.1.14;
DE Includes:
DE RecName: Full=Glycine--tRNA ligase alpha subunit;
DE AltName: Full=Glycyl-tRNA synthetase alpha subunit;
DE Includes:
DE RecName: Full=Glycine--tRNA ligase beta subunit;
DE AltName: Full=Glycyl-tRNA synthetase beta subunit;
GN Name=glyQS; Synonyms=glyQ; OrderedLocusNames=CPn_0946, CP_0913, CpB0981;
OS Chlamydia pneumoniae (Chlamydophila pneumoniae).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=83558;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CWL029;
RX PubMed=10192388; DOI=10.1038/7716;
RA Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W.,
RA Olinger L., Grimwood J., Davis R.W., Stephens R.S.;
RT "Comparative genomes of Chlamydia pneumoniae and C. trachomatis.";
RL Nat. Genet. 21:385-389(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AR39;
RX PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA Salzberg S.L., Eisen J.A., Fraser C.M.;
RT "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT AR39.";
RL Nucleic Acids Res. 28:1397-1406(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J138;
RX PubMed=10871362; DOI=10.1093/nar/28.12.2311;
RA Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K.,
RA Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.;
RT "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from
RT Japan and CWL029 from USA.";
RL Nucleic Acids Res. 28:2311-2314(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TW-183;
RA Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P.,
RA Schneider S., Pohl T., Essig A., Marre R., Melchers K.;
RT "The genome sequence of Chlamydia pneumoniae TW183 and comparison with
RT other Chlamydia strains based on whole genome sequence analysis.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD19084.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA99154.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE001363; AAD19084.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE002161; AAF38698.1; -; Genomic_DNA.
DR EMBL; BA000008; BAA99154.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE009440; AAP98910.1; -; Genomic_DNA.
DR PIR; A72016; A72016.
DR PIR; C81524; C81524.
DR PIR; H86608; H86608.
DR RefSeq; NP_225141.1; NC_000922.1.
DR AlphaFoldDB; Q9Z6W0; -.
DR SMR; Q9Z6W0; -.
DR STRING; 115711.CP_0913; -.
DR EnsemblBacteria; AAD19084; AAD19084; CPn_0946.
DR EnsemblBacteria; AAF38698; AAF38698; CP_0913.
DR KEGG; cpa:CP_0913; -.
DR KEGG; cpj:glyQ; -.
DR KEGG; cpn:CPn_0946; -.
DR KEGG; cpt:CpB0981; -.
DR PATRIC; fig|115713.3.peg.1035; -.
DR eggNOG; COG0751; Bacteria.
DR eggNOG; COG0752; Bacteria.
DR HOGENOM; CLU_007220_1_1_0; -.
DR Proteomes; UP000000583; Chromosome.
DR Proteomes; UP000000801; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; ISS:UniProtKB.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00733; GlyRS_alpha_core; 1.
DR Gene3D; 3.30.930.10; -; 1.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR PANTHER; PTHR30075; PTHR30075; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00388; glyQ; 1.
DR TIGRFAMs; TIGR00211; glyS; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..1010
FT /note="Glycine--tRNA ligase"
FT /id="PRO_0000072889"
FT REGION 1..312
FT /note="Glycine--tRNA ligase alpha subunit"
FT REGION 313..1010
FT /note="Glycine--tRNA ligase beta subunit"
FT CONFLICT 177
FT /note="T -> I (in Ref. 1; AAD19084)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1010 AA; 114032 MW; 2B63999426DC195C CRC64;
MSEHPLTLQS MIATILRFWS EQGCVIHQGY DLEVGAGTFN PATFLRALGP EPYKAAYVEP
SRRPQDGRYG VHPNRLQNYH QLQVILKPVP ENFLSLYTES LRAIGLDLRD HDIRFIHDDW
ENPTIGAWGL GWEVWLNGME ITQLTYFQAI GSKPLDTISG EITYGIERIA MYLQKKTSIY
DVLWNDTLTY GQITQASEKA WSEYNFDYAN TEMWFKHFED FAEEALRTLK NGLSVPAYDF
VIKASHAFNI LDARGTISVT ERTRYIARIR QLTRLVADSY VEWRASLNYP LLSLSSTSEP
KETSESVVPM ISSTEDLLLE IGSEELPATF VPIGIQQLES LARQVLTDHN IVYEGLEVLG
SPRRLALLVK NVAPEVVQKA FEKKGPMLTS LFSPDGDVSP QGQQFFASQG VDISHYQDLS
RHASLAIRTV NGSEYLFLLH PEIRLRTADI LMQELPLLIQ RMKFPKKMVW DNSGVEYARP
IRWLVALYGE HILPITLGTI IASRNSFGHR QLDPRKISIS SPQDYVETLR QACVVVSQKE
RRMIIEQGLR AHSSDTISAI PLPRLIEEAT FLSEHPFVSC GQFSEQFCAL PKELLIAEMV
NHQKYFPTHE TSSGAISNFF IVVCDNSPND TIIEGNEKAL TPRLTDGEFL FKQDLQTPLT
TFIEKLKSVT YFEALGSLYD KVERLKAHQR VFSTFSSLAA SEDLDIAIQY CKADLVSAVV
NEFPELQGIM GEYYLKHANL PTASAVAVGE HLRHITMGQK LSTIGTLLSL LDRLDNLLAC
FILGLKPTSS HDPYALRRQS LEVLTLVSAS RLPIDLASLL DRLADHFPST IEEKVWDKSK
TIHEILEFIW GRLKTFMGSL EFRKDEIAAV LIDSATKNPI EILDTAEALQ LLKEEHTEKL
AVITTTHNRL KKILSSLKLS MTSSPIEVLG DRESNFKQVL DAFPGFPKET SAHAFLEYFL
SLADLSNDIQ DFLNTVHIAN DDGAIRNLRI SLLLTAMDKF SLCHWESVAV
