ID   SYH2_BACCR              Reviewed;         423 AA.
AC   Q817X7;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Histidine--tRNA ligase 2 {ECO:0000255|HAMAP-Rule:MF_00127};
DE            EC=6.1.1.21 {ECO:0000255|HAMAP-Rule:MF_00127};
DE   AltName: Full=Histidyl-tRNA synthetase 2 {ECO:0000255|HAMAP-Rule:MF_00127};
DE            Short=HisRS 2 {ECO:0000255|HAMAP-Rule:MF_00127};
GN   Name=hisS-2 {ECO:0000255|HAMAP-Rule:MF_00127}; OrderedLocusNames=BC_4398;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-
CC         histidyl-tRNA(His); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC         Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00127};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00127}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00127}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00127}.
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DR   EMBL; AE016877; AAP11311.1; -; Genomic_DNA.
DR   RefSeq; NP_834110.1; NC_004722.1.
DR   RefSeq; WP_000027084.1; NZ_CP034551.1.
DR   AlphaFoldDB; Q817X7; -.
DR   SMR; Q817X7; -.
DR   STRING; 226900.BC_4398; -.
DR   EnsemblBacteria; AAP11311; AAP11311; BC_4398.
DR   GeneID; 67509040; -.
DR   KEGG; bce:BC4398; -.
DR   PATRIC; fig|226900.8.peg.4549; -.
DR   HOGENOM; CLU_025113_1_1_9; -.
DR   OMA; CDFDFIG; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004821; F:histidine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006427; P:histidyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00773; HisRS-like_core; 1.
DR   CDD; cd00859; HisRS_anticodon; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   HAMAP; MF_00127; His_tRNA_synth; 1.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR015807; His-tRNA-ligase.
DR   InterPro; IPR041715; HisRS-like_core.
DR   InterPro; IPR004516; HisRS/HisZ.
DR   InterPro; IPR033656; HisRS_anticodon.
DR   PANTHER; PTHR43707; PTHR43707; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF13393; tRNA-synt_His; 1.
DR   PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00442; hisS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..423
FT                   /note="Histidine--tRNA ligase 2"
FT                   /id="PRO_0000136098"
SQ   SEQUENCE   423 AA;  47763 MW;  E12671159D0E92CF CRC64;
     MSIQIPRGTQ DILPGTVELW QYIEGQAREI CRRYNYKEIR TPIFEHTELF LRGVGDTTDI
     VQKEMYSFQD RGERSLTLRP EGTAPVVRSY VENKMFGDAT QPTKLYYIGQ MFRYERPQAG
     RYRQFVQFGI EAIGSNDPAI DAEVIALAVE FYRGMGLKNI KVVLNSLGDA ASRQAHRDAL
     IAHFEPRIGE FCSDCQSRLE KNPLRILDCK KDRNHELMGT APSITEYLNE DSAVYYDKVQ
     ELLTMMDVPF EKDPNLVRGL DYYQHTVFEI MSEAEGFGAI TTLSGGGRYN GLVQEIGGPE
     MPGIGFAMSI ERLIMALKAE NIELPLEHSI DCYVVALGEK AKDHAAKVAF DLRKAGLSVE
     KDYLDRKMKA QFKSADRLKA KFVAVLGEDE LDKGIINLKD MATGEQEEVA LDVFASYVAE
     KLI