ID   BLA1_ENTCL              Reviewed;         286 AA.
AC   P18251;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Beta-lactamase Ohio-1;
DE            EC=3.5.2.6;
DE   Flags: Precursor;
OS   Enterobacter cloacae.
OG   Plasmid pDS075.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2121093; DOI=10.1128/aac.34.8.1570;
RA   Shlaes D.M., Currie-Mccumber C., Hull A., Behlau I., Kron M.;
RT   "OHIO-1 beta-lactamase is part of the SHV-1 family.";
RL   Antimicrob. Agents Chemother. 34:1570-1576(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10101};
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC       {ECO:0000305}.
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DR   EMBL; M33655; AAA72078.1; -; Unassigned_DNA.
DR   PIR; A44958; A44958.
DR   AlphaFoldDB; P18251; -.
DR   SMR; P18251; -.
DR   DrugBank; DB01606; Tazobactam.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR045155; Beta-lactam_cat.
DR   InterPro; IPR000871; Beta-lactam_class-A.
DR   InterPro; IPR023650; Beta-lactam_class-A_AS.
DR   PANTHER; PTHR35333; PTHR35333; 1.
DR   Pfam; PF13354; Beta-lactamase2; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Disulfide bond; Hydrolase; Plasmid; Signal.
FT   SIGNAL          1..21
FT   CHAIN           22..286
FT                   /note="Beta-lactamase Ohio-1"
FT                   /id="PRO_0000016996"
FT   ACT_SITE        66
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         230..232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..119
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   286 AA;  31354 MW;  9FE16709361AF9C5 CRC64;
     MRYFRLCIIS LLATLPLRVH AGPQPLEQIK LSESQLSGSV GMIEMDLARP GTLTAWRADE
     RFPMMSTFKV VLCGAGLARV DAGDEQLERK IHYRRQDLVD YSPVSEKHLA DGMTVGELCA
     AAITMSDNSA ANLLLPAVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTAR
     SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGRLIRSV LPAGWFIADK TGAGERGARG
     IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIAGAL IEHWQR