BLA34_ECOLX
ID BLA34_ECOLX Reviewed; 286 AA.
AC Q93LM8;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Beta-lactamase SHV-34;
DE EC=3.5.2.6;
DE Flags: Precursor;
GN Name=bla; Synonyms=shv34;
OS Escherichia coli.
OG Plasmid pOZ185.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE FUNCTION.
RX PubMed=14613945; DOI=10.1093/jac/dkh017;
RA Heritage J., Chambers P.A., Tyndall C., Buescher E.S.;
RT "SHV-34: an extended-spectrum beta-lactamase encoded by an epidemic
RT plasmid.";
RL J. Antimicrob. Chemother. 52:1015-1017(2003).
CC -!- FUNCTION: Hydrolyzes ceftazidime and cefotaxime. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10101};
CC -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC {ECO:0000305}.
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DR EMBL; AY036620; AAK64187.1; -; Genomic_DNA.
DR RefSeq; WP_063864676.1; NG_050073.1.
DR AlphaFoldDB; Q93LM8; -.
DR SMR; Q93LM8; -.
DR KEGG; ag:AAK64187; -.
DR GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 3.40.710.10; -; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR045155; Beta-lactam_cat.
DR InterPro; IPR000871; Beta-lactam_class-A.
DR InterPro; IPR023650; Beta-lactam_class-A_AS.
DR PANTHER; PTHR35333; PTHR35333; 1.
DR Pfam; PF13354; Beta-lactamase2; 1.
DR PRINTS; PR00118; BLACTAMASEA.
DR SUPFAM; SSF56601; SSF56601; 1.
DR PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Disulfide bond; Hydrolase; Plasmid; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..286
FT /note="Beta-lactamase SHV-34"
FT /id="PRO_0000352523"
FT ACT_SITE 66
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT ACT_SITE 164
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 230..232
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT DISULFID 73..119
FT /evidence="ECO:0000250"
SQ SEQUENCE 286 AA; 31147 MW; D34628D71B552FD4 CRC64;
MRYFRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGSV GMIEMDLASG RTLTAWRADG
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR