ID   BLA6_KLEPN              Reviewed;         260 AA.
AC   P96348;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Beta-lactamase SHV-6;
DE            EC=3.5.2.6;
DE   Flags: Precursor; Fragment;
GN   Name=bla; Synonyms=shv6;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SLK-47;
RX   PubMed=9228783; DOI=10.1016/s0378-1097(97)00196-1;
RA   Arlet G., Rouveau M., Philippon A.;
RT   "Substitution of alanine for aspartate at position 179 in the SHV-6
RT   extended-spectrum beta-lactamase.";
RL   FEMS Microbiol. Lett. 152:163-167(1997).
CC   -!- FUNCTION: SHV enzymes hydrolyze broad spectrum cephalosporins notably
CC       cefotaxime and ceftazidime.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10101};
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC       {ECO:0000305}.
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DR   EMBL; Y11069; CAA71948.1; -; Genomic_DNA.
DR   AlphaFoldDB; P96348; -.
DR   SMR; P96348; -.
DR   KEGG; ag:CAA71948; -.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR045155; Beta-lactam_cat.
DR   InterPro; IPR000871; Beta-lactam_class-A.
DR   InterPro; IPR023650; Beta-lactam_class-A_AS.
DR   PANTHER; PTHR35333; PTHR35333; 1.
DR   Pfam; PF13354; Beta-lactamase2; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Disulfide bond; Hydrolase; Signal.
FT   SIGNAL          <1..11
FT                   /evidence="ECO:0000255"
FT   CHAIN           12..>260
FT                   /note="Beta-lactamase SHV-6"
FT                   /id="PRO_0000016985"
FT   ACT_SITE        56
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         220..222
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   DISULFID        63..109
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         260
SQ   SEQUENCE   260 AA;  28157 MW;  26A8E2D43632EAA0 CRC64;
     LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE RFPMMSTFKV
     VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA AAITMSDNSA
     ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARATTTPA SMAATLRKLL
     TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK
     AERIVVIYLR DTPASMAERN