ID   SYH_MYCLE               Reviewed;         427 AA.
AC   P46696; O69488;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=Histidine--tRNA ligase;
DE            EC=6.1.1.21;
DE   AltName: Full=Histidyl-tRNA synthetase;
DE            Short=HisRS;
GN   Name=hisS; OrderedLocusNames=ML0494; ORFNames=B1177_C3_248, MLCB1259.12;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-
CC         histidyl-tRNA(His); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC         Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; U00011; AAA17103.1; -; Genomic_DNA.
DR   EMBL; AL023591; CAA19087.1; -; Genomic_DNA.
DR   EMBL; AL583918; CAC30002.1; -; Genomic_DNA.
DR   PIR; F86970; F86970.
DR   PIR; S72739; S72739.
DR   RefSeq; NP_301433.1; NC_002677.1.
DR   RefSeq; WP_010907757.1; NC_002677.1.
DR   AlphaFoldDB; P46696; -.
DR   SMR; P46696; -.
DR   STRING; 272631.ML0494; -.
DR   EnsemblBacteria; CAC30002; CAC30002; CAC30002.
DR   KEGG; mle:ML0494; -.
DR   PATRIC; fig|272631.5.peg.858; -.
DR   Leproma; ML0494; -.
DR   eggNOG; COG0124; Bacteria.
DR   HOGENOM; CLU_025113_1_1_11; -.
DR   OMA; CDFDFIG; -.
DR   BRENDA; 6.1.1.21; 3504.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004821; F:histidine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006427; P:histidyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00773; HisRS-like_core; 1.
DR   CDD; cd00859; HisRS_anticodon; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   HAMAP; MF_00127; His_tRNA_synth; 1.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR015807; His-tRNA-ligase.
DR   InterPro; IPR041715; HisRS-like_core.
DR   InterPro; IPR004516; HisRS/HisZ.
DR   InterPro; IPR033656; HisRS_anticodon.
DR   PANTHER; PTHR43707; PTHR43707; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF13393; tRNA-synt_His; 1.
DR   PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00442; hisS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..427
FT                   /note="Histidine--tRNA ligase"
FT                   /id="PRO_0000136194"
FT   CONFLICT        41
FT                   /note="A -> R (in Ref. 1; AAA17103)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   427 AA;  45694 MW;  4FA645DFFB7D5405 CRC64;
     MTESCTVFSF SGPKGIPDYF PPDSAQFVAV RDGLLTAARR AGYGDIELPV FEDTALFARG
     VGESTDVVAK EMYTFADRGD RSVTLRPEGT AGVVRAVIEH GLDRGALPVK LCYAGPFFRY
     ERPQAGRCRQ LQQVGVEAIG VDDPALDAEV ITIADAGFRS LGLDGFQLEI TSLGDGTCRP
     QYRKLLQEFL LQLDLDEDTR RRAELNPLRV LDDKRPQVQA MTAAAPVLLD HLSDGAKQHF
     DTVLAHLDAL RVPYVINPRM VRGLDYYTKT TFEFVHPGLG AQSGIGGGGR YDGLMRQLGG
     QDLSGIGFGL GVDRTLLALH AEGKTVGETT RCDVFGVSLG EAAKLKVAML AGQLRAAGVR
     VDLIYGDRGI RGAMRAAGRS GARIALIVDD CAIKADGVGV RDLATGEQIS VAVDSVVAEV
     ISRIAPS