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BLAB_PROVU
ID   BLAB_PROVU              Reviewed;         300 AA.
AC   P52664;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Beta-lactamase;
DE            EC=3.5.2.6;
DE   AltName: Full=Cefuroximase;
DE   Flags: Precursor;
GN   Name=blaB; Synonyms=cumA;
OS   Proteus vulgaris.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Proteus.
OX   NCBI_TaxID=585;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B317;
RX   PubMed=7957242; DOI=10.1111/j.1432-1033.1994.tb20036.x;
RA   Datz M., Joris B., Azab E.A., Galleni M., van Beeumen J., Frere J.-M.,
RA   Martin H.H.;
RT   "A common system controls the induction of very different genes. The class-
RT   A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-
RT   lactamase.";
RL   Eur. J. Biochem. 226:149-157(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=5E78-1;
RA   Okuguchi M., Komai T., Makajima N., Eguchi H., Kuboki A., Ito T.,
RA   Meguro M., Nakata T., Sugimoto K.;
RL   Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hydrolyzes broad-spectrum beta-lactam antibiotics. Active
CC       against cephalosporins such as cefuroxime and cefotaxime.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10101};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC       {ECO:0000305}.
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DR   EMBL; X80128; CAA56427.1; -; Genomic_DNA.
DR   EMBL; D37831; BAA07084.1; -; Genomic_DNA.
DR   PIR; S51044; S51044.
DR   RefSeq; WP_063851288.1; NG_047908.1.
DR   PDB; 1HZO; X-ray; 1.75 A; A/B=32-300.
DR   PDBsum; 1HZO; -.
DR   AlphaFoldDB; P52664; -.
DR   SMR; P52664; -.
DR   DrugBank; DB03814; 2-(N-morpholino)ethanesulfonic acid.
DR   EvolutionaryTrace; P52664; -.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR045155; Beta-lactam_cat.
DR   InterPro; IPR000871; Beta-lactam_class-A.
DR   InterPro; IPR023650; Beta-lactam_class-A_AS.
DR   PANTHER; PTHR35333; PTHR35333; 1.
DR   Pfam; PF13354; Beta-lactamase2; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Hydrolase; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..300
FT                   /note="Beta-lactamase"
FT                   /id="PRO_0000017008"
FT   ACT_SITE        75
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT   BINDING         239..241
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   VARIANT         13
FT                   /note="T -> A (in strain: 5E78-1)"
FT   VARIANT         23
FT                   /note="V -> A (in strain: 5E78-1)"
FT   VARIANT         30..31
FT                   /note="NT -> DN (in strain: 5E78-1)"
FT   VARIANT         40
FT                   /note="S -> N (in strain: 5E78-1)"
FT   VARIANT         58
FT                   /note="E -> K (in strain: 5E78-1)"
FT   VARIANT         88
FT                   /note="E -> A (in strain: 5E78-1)"
FT   VARIANT         119
FT                   /note="V -> T (in strain: 5E78-1)"
FT   VARIANT         123
FT                   /note="S -> T (in strain: 5E78-1)"
FT   VARIANT         126
FT                   /note="Q -> E (in strain: 5E78-1)"
FT   VARIANT         224
FT                   /note="H -> N (in strain: 5E78-1)"
FT   VARIANT         235
FT                   /note="I -> V (in strain: 5E78-1)"
FT   VARIANT         257
FT                   /note="K -> E (in strain: 5E78-1)"
FT   VARIANT         283
FT                   /note="V -> A (in strain: 5E78-1)"
FT   VARIANT         286
FT                   /note="T -> A (in strain: 5E78-1)"
FT   HELIX           34..46
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          48..56
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   TURN            57..60
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          61..66
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           74..77
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           78..91
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           95..97
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           104..106
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           114..116
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   TURN            118..120
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           124..133
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           137..147
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           150..159
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           173..175
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           188..199
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          201..204
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           206..217
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   TURN            223..225
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           226..229
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          234..243
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   TURN            244..246
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          247..255
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   STRAND          262..269
FT                   /evidence="ECO:0007829|PDB:1HZO"
FT   HELIX           279..294
FT                   /evidence="ECO:0007829|PDB:1HZO"
SQ   SEQUENCE   300 AA;  32992 MW;  2719AF2464F1CD77 CRC64;
     MTMFKTTFRQ TATIAVSLIS LLVSPMLWAN TNNTIEEQLS TLEKYSQGRL GVALINTEDN
     SQITYRGEER FAMASTSKVM AVAAVLKESE KQAGLLDKNI TIKKSDLVAY SPITEKHLVT
     GMSLAQLSAA TLQYSDNTAM NKILDYLGGP AKVTQFARSI NDVTYRLDRK EPELNTAIHG
     DPRDTTSPIA MAKSLQALTL GDALGQSQRQ QLVTWLKGNT TGDHSIKAGL PKHWIVGDKT
     GSGDYGTTND IAVIWPKNHA PLILVVYFTQ QEQDAKYRKD IIVKATEIVT KEISNSPQTK
 
 
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