SYH_THEFY
ID SYH_THEFY Reviewed; 438 AA.
AC Q47RX0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Histidine--tRNA ligase;
DE EC=6.1.1.21;
DE AltName: Full=Histidyl-tRNA synthetase;
DE Short=HisRS;
GN Name=hisS; OrderedLocusNames=Tfu_0759;
OS Thermobifida fusca (strain YX).
OC Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
OC Thermobifida.
OX NCBI_TaxID=269800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YX;
RX PubMed=17209016; DOI=10.1128/jb.01899-06;
RA Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G.,
RA Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B.,
RA Kyrpides N.;
RT "Genome sequence and analysis of the soil cellulolytic actinomycete
RT Thermobifida fusca YX.";
RL J. Bacteriol. 189:2477-2486(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-
CC histidyl-tRNA(His); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000088; AAZ54797.1; -; Genomic_DNA.
DR RefSeq; WP_011291206.1; NC_007333.1.
DR AlphaFoldDB; Q47RX0; -.
DR SMR; Q47RX0; -.
DR STRING; 269800.Tfu_0759; -.
DR PRIDE; Q47RX0; -.
DR EnsemblBacteria; AAZ54797; AAZ54797; Tfu_0759.
DR KEGG; tfu:Tfu_0759; -.
DR eggNOG; COG0124; Bacteria.
DR HOGENOM; CLU_025113_3_0_11; -.
DR OMA; YQIQKVW; -.
DR OrthoDB; 277998at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004821; F:histidine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00773; HisRS-like_core; 1.
DR Gene3D; 3.30.930.10; -; 1.
DR Gene3D; 3.40.50.800; -; 1.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004154; Anticodon-bd.
DR InterPro; IPR036621; Anticodon-bd_dom_sf.
DR InterPro; IPR015807; His-tRNA-ligase.
DR InterPro; IPR041715; HisRS-like_core.
DR InterPro; IPR004516; HisRS/HisZ.
DR Pfam; PF03129; HGTP_anticodon; 1.
DR Pfam; PF13393; tRNA-synt_His; 1.
DR PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00442; hisS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..438
FT /note="Histidine--tRNA ligase"
FT /id="PRO_0000136281"
SQ SEQUENCE 438 AA; 48660 MW; FDD26DA4C5A814F3 CRC64;
MSEQRIIRPT PISGFPEWTP RIRSVELRWL DHIRRGFERY GFSSVETPSV EVLDVLLSKG
ETSQEIYTLQ RLQADADDSS DARLGLHFDL TVPFARYVAQ HFNDLVFPFK RYQIQRVWRG
ERPQEGRFRE FTQCDIDVIN VDRIPLHFDA ELPRIVHEVL TGLDIPAWTL NINNRKVLQG
FYEGLGITDP LAVIRAVDKL HKIGADAVRE ILIDQAGLSS DQAAACLELA EIRGSDTTVV
DAVAKLGVSH PTLTAGLDEL GEVLDELSDL PSGSVVADLS IARGLDYYTG TVYEATFNDD
PGYGSICAGG RYENLAGQFI RRSLPGVGIS IGLTRIFAKL VSEGRITGGR FCPTDVLVVL
PSDERRSAAL AVAAQLRERG FNTEVYHQAA KIGKQIQYAV KKDIPFVWFP PFDDGRPHEV
KNLATGEQVE ADPASWNG