SYIC_ARATH
ID SYIC_ARATH Reviewed; 1190 AA.
AC F4JLM5; O82613; Q9SV89;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Isoleucine--tRNA ligase, cytoplasmic {ECO:0000305};
DE EC=6.1.1.5 {ECO:0000305};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000305};
DE Short=IleRS {ECO:0000305};
GN OrderedLocusNames=At4g10320 {ECO:0000312|Araport:AT4G10320};
GN ORFNames=F24G24.120 {ECO:0000312|EMBL:CAB39785.1},
GN T9A4.4 {ECO:0000312|EMBL:AAC62806.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 782-1190.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=16297076; DOI=10.1111/j.1365-313x.2005.02580.x;
RA Berg M., Rogers R., Muralla R., Meinke D.;
RT "Requirement of aminoacyl-tRNA synthetases for gametogenesis and embryo
RT development in Arabidopsis.";
RL Plant J. 44:866-878(2005).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=16251277; DOI=10.1073/pnas.0504682102;
RA Duchene A.-M., Giritch A., Hoffmann B., Cognat V., Lancelin D.,
RA Peeters N.M., Zaepfel M., Marechal-Drouard L., Small I.D.;
RT "Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in
RT Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:16484-16489(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000305|PubMed:16251277,
CC ECO:0000305|PubMed:16297076}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC62806.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB39785.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78155.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF096373; AAC62806.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL049488; CAB39785.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161517; CAB78155.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82867.1; -; Genomic_DNA.
DR EMBL; BX827114; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T01968; T01968.
DR PIR; T04047; T04047.
DR RefSeq; NP_192770.2; NM_117100.4.
DR AlphaFoldDB; F4JLM5; -.
DR SMR; F4JLM5; -.
DR IntAct; F4JLM5; 1.
DR STRING; 3702.AT4G10320.1; -.
DR PaxDb; F4JLM5; -.
DR PRIDE; F4JLM5; -.
DR ProteomicsDB; 234105; -.
DR EnsemblPlants; AT4G10320.1; AT4G10320.1; AT4G10320.
DR GeneID; 826624; -.
DR Gramene; AT4G10320.1; AT4G10320.1; AT4G10320.
DR KEGG; ath:AT4G10320; -.
DR Araport; AT4G10320; -.
DR TAIR; locus:2122764; AT4G10320.
DR eggNOG; KOG0434; Eukaryota.
DR HOGENOM; CLU_001493_1_1_1; -.
DR InParanoid; F4JLM5; -.
DR OMA; VHYCSIP; -.
DR OrthoDB; 59501at2759; -.
DR PRO; PR:F4JLM5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JLM5; baseline and differential.
DR Genevisible; F4JLM5; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR42780; PTHR42780; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00392; ileS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 2: Evidence at transcript level;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..1190
FT /note="Isoleucine--tRNA ligase, cytoplasmic"
FT /id="PRO_0000433536"
FT REGION 271..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 49..59
FT /note="'HIGH' region"
FT /evidence="ECO:0000305"
FT MOTIF 632..636
FT /note="'KMSKS' region"
FT /evidence="ECO:0000305"
FT BINDING 635
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1190 AA; 135484 MW; 6DFE30B4D6300F73 CRC64;
MEEVCEGKEF SFPRQEEDVL SFWTEIDAFK TQLKRTENLP EYIFYDGPPF ATGLPHYGHI
LAGTIKDIVT RYQTMTGHHV TRRFGWDCHG LPVENEIDRK LNIKRRDEVI KMGIDKYNEE
CRSIVTRYVA EWEKVITRCG RWIDFKNDYK TMDLPFMESV WWVFSQLWEK NLVYRGFKVM
PYSTGCKTPL SNFEAGQNYK EVPDPEIMVT FPVIGDQDNA AFVAWTTTPW TLPSNLALCV
NAKFVYVKVR NKNTGKVYIV AESRLSALPT DKPKAKLSNG PAGDTKKANP KAKGAKPESA
ADSYEVLEKF NGASLVGKKY EPLFDYFSDF SSEAFRVVAD DYVTDDSGTG IVHCAPAFGE
DDYRVCLLNK IIKKGENLVV AVDDDGLFTE RITHFSGRYV KDADKDIIEA VKAKGRLVKT
GSFTHSYPFC WRSDTPLIYR AVPSWFVRVE QLKEKLLKSN EQTEWVPGYV KDKRFHNWLE
NARDWAISRS RFWGTPLPIW ISDDGEEVVI MDSVEKLEKL SGVKVFDLHR HHIDHITIPS
SRGDEFGVLR RVEDVFDCWF ESGSMPYAYI HYPFENKELF EKNFPGDFVA EGLDQTRGWF
YTLMVLSTAL FEKPAFKNLI CNGLVLAEDG KKMAKKLRNY PPPLEVIDEY GADAVRLYLI
NSPVVRAEPL RFKKEGVLGV VKDVFLPWYN AYRFLVQNAK RLETEGGVPF VPTDLATIQS
ANILDQWIHS ATQSLVRFVR EEMDAYRLYT VVPRLLKFLD NLTNIYVRFN RKRLKGRTGE
DDCHTALSTL FNVLLTSCKV MAPFTPFFTE TLYQNLRKAC KGSEESVHYC SIPPREGMEG
ERIELSVTRM MKIIDLARNI RERNKLPLKT PLKEMIVVHP DADFLNDITG VLREYVLEEL
NVRSLVPCND TLKYASLKAE PDFSVLGKRL GKSMGLVAKE VKEMSQKDIL AFEEAGEVTI
ANHLLKETDI KIVRVFKRPD DLKENEIDSA GDGDVLVILD LRADDSLVEA GFAREIVNRI
QKLRKKSGLE PTDFVEVYFQ SLDEDESVSK QVLVSQEQNI KDSIGSTLLL SSLMPSHAVI
IADETFTPKE TSDESVKKVP KLSYKISLAR PALKFNEEAV LALYSGDVKS ATGLQTYLLS
RDHSNLKSEF QAGDGKITVS CIENVPVATV VLGEHLHLSV GDDLLSKRNA
