ID   SYIC_CAEEL              Reviewed;        1141 AA.
AC   Q21926;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Isoleucine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
GN   Name=iars-1 {ECO:0000312|WormBase:R11A8.6};
GN   Synonyms=irs-1 {ECO:0000312|WormBase:R11A8.6};
GN   ORFNames=R11A8.6 {ECO:0000312|WormBase:R11A8.6};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; Z70310; CAA94369.1; -; Genomic_DNA.
DR   PIR; T24176; T24176.
DR   RefSeq; NP_501914.1; NM_069513.4.
DR   AlphaFoldDB; Q21926; -.
DR   SMR; Q21926; -.
DR   BioGRID; 43028; 15.
DR   IntAct; Q21926; 1.
DR   MINT; Q21926; -.
DR   STRING; 6239.R11A8.6.1; -.
DR   EPD; Q21926; -.
DR   PaxDb; Q21926; -.
DR   PeptideAtlas; Q21926; -.
DR   EnsemblMetazoa; R11A8.6.1; R11A8.6.1; WBGene00002152.
DR   EnsemblMetazoa; R11A8.6.2; R11A8.6.2; WBGene00002152.
DR   UCSC; R11A8.6; c. elegans.
DR   WormBase; R11A8.6; CE06304; WBGene00002152; iars-1.
DR   eggNOG; KOG0434; Eukaryota.
DR   GeneTree; ENSGT00550000074921; -.
DR   HOGENOM; CLU_001493_1_1_1; -.
DR   InParanoid; Q21926; -.
DR   OMA; KMMAPFT; -.
DR   OrthoDB; 59501at2759; -.
DR   PhylomeDB; Q21926; -.
DR   PRO; PR:Q21926; -.
DR   Proteomes; UP000001940; Chromosome IV.
DR   Bgee; WBGene00002152; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033709; Anticodon_Ile_ABEc.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR42780; PTHR42780; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00392; ileS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..1141
FT                   /note="Isoleucine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000098599"
FT   MOTIF           50..60
FT                   /note="'HIGH' region"
FT   MOTIF           601..605
FT                   /note="'KMSKS' region"
FT   BINDING         604
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1141 AA;  130012 MW;  151E6AD6D6360EB0 CRC64;
     MSGLSTVPDN INFAREEDKV AQKWQDENTF QRSVELSKDR PHFTFYDGPP FATGLPHYGH
     MLTSTIKDVV GRWAHQNGHY VERRFGWDTH GLPVEYEIDK TLGISGPQDV MKMGIANYNN
     ECRKIVMRYS GEWEKTMGRL GRWVDFKHDY KTLYPWFMES VWWAFSELHK KGLVYKGVKV
     MPFSTACSTP LSNFEAGQNY KDVVDPAVFV GFKLLDCPNR QLVAWTTTPW TLPSNLALVV
     HPDMLYVVTK DKTTGIEYVV LEERLGELKN DNLEVIEKLA GSQLKDLRYE PLFPYFAYMR
     EERNAFRVLN DTFVTSDSGT GVVHQAPYFG EIDFQVCVAN GVIAKDQKMI CPVDESGKYT
     SEVPDYQGVY VKDADKLIIK RLKEMGNLVR QAEVKHSYPF CWRSDTPLLY KAVPSWFINV
     ETLIPRLLAN NDETYWVPAF VKEKRFANWL RDARDWAVSR NRFWGTPINL WVSEDGEEVV
     CVGSIAELEE LSGQKITDLH RESVDDVTIP SRSGRGVLKR VSEVFDCWFE SGSMPYAQNH
     YPFENRKIFE DNFPADFIAE GIDQTRGWFY TLLVLSTALF NKPPFKNLIC NGLVLASDGA
     KMSKSKKNYP DPMLIVNKYG ADALRLYLIN SPVVRGENLR FREEGVRDLL KDVFLPWFNA
     YRFFVQNVQA YEHETGNVFD MNVHVASENV MDRWIESFTN SLVAFVRKEM DSYRLYAVVG
     PLTKFFDTLT NIYIRLNRKR VKGDNGLHEQ HHALAALGRV LILIVRLMAP FTPFFCEYIW
     LNLKKVIGST EESVHFLMLP KPDESLIDET VERRVEVMRN VIDLVRLVRD REGLAVKYPL
     KEMIVINRDS QFLEDVKSLE SYILLELNVR KLTVSQDKQK YGITLKAEPN FKILGARLKG
     EQKKVADYLK NQITESELEK FLLEGKLTVL GHELSSEEVA VSYAAGSDQG HGYKTHSDAK
     TIVMIDTTED ESLIEEGLCR EVTNRVQRLR KQAKLVSTDT AHVHIVVSPA DSQLAKVVAA
     KLNDIVSATG TPIKLGSPPA AAKAPTATSK SAIKDSEVEL WLFAEGDSFD GITVVDGSKK
     VRVHVKTEKE HLKGYADLLY HVRSALDLWN GKIALKNVDG SAVHPTVDVT SLVGQTLQLV
     R