ID   SYIC_ENTBH              Reviewed;         953 AA.
AC   B7XK16;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Probable isoleucine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
GN   ORFNames=EBI_22873;
OS   Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC   Enterocytozoon.
OX   NCBI_TaxID=481877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA   Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA   Keeling P.J.;
RT   "Patterns of genome evolution among the microsporidian parasites
RT   Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT   bieneusi.";
RL   PLoS ONE 2:E1277-E1277(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA   Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA   Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT   "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT   Enterocytozoon bieneusi.";
RL   PLoS Pathog. 5:E1000261-E1000261(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ABGB01000039; EED43719.1; -; Genomic_DNA.
DR   RefSeq; XP_002650344.1; XM_002650298.1.
DR   AlphaFoldDB; B7XK16; -.
DR   SMR; B7XK16; -.
DR   STRING; 481877.B7XK16; -.
DR   EnsemblFungi; EED43719; EED43719; EBI_22873.
DR   VEuPathDB; MicrosporidiaDB:EBI_22873; -.
DR   HOGENOM; CLU_001493_1_1_1; -.
DR   InParanoid; B7XK16; -.
DR   Proteomes; UP000001742; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR42780; PTHR42780; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00392; ileS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..953
FT                   /note="Probable isoleucine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388379"
FT   MOTIF           45..55
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           634..638
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         637
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   953 AA;  112077 MW;  821531819140DFB2 CRC64;
     MSDKIIDFSK IEKDILKFWT ENKIFELCNK LSQGREEYVF FDGPPFATGL PHYGHILSGT
     IKDIIGRFYY QKGFHVERRF GWDCHGLPVE YEIDKKFNIT DRNQILKMGI DVYNHECRKI
     VQKYTNEWEE TVIRMGRWVD FKGSYKTMDL EFMESIWFIF KELFKRNKIY RGFKIMPFST
     ACKTALSNFE ASQNYKNVSD PSVLVTFKII NPKDVLHNYE NNDIEIFFVA WTTTPWTLPA
     NCALVINPDY EYAIFYINNN SKKLYIMLKN RIEVYFKQFC ITTVILGKEL IGVSYATLFN
     YYENYITRGF FKTIGGNFVT ITDGSGIVHT APAFGEEDYN IFLKLGLLKP DEIPPCHIDE
     NGKFTIAMEK YVNVPINNDI SLNGVYFKDA DKIILQILKP HLIYNSRIVH SYPFCWRSDT
     PLLYKLIPNW FVKITDIREE LLTQNKKINW IPETIKYKRF QNWLSNAKDW AISRNRFWGT
     PLPIWARYEN EIYNYTDLIC VGSIKELEDL TNTKITDLHR EHIDQLLIEH NGKTYKRIED
     VLDCWFESGS MPYAQNHLKG IKVNNSLIDK LDNINLNNAY LIKENFPADF IGEGLDQTRG
     WFYTLHVIST ILFNSPAFKN VIVNGIVLAE DGKKMSKRLK NYPDPMNIFN KYGADALRMY
     LISSPVVEAE NLRFNENGVK EILKTLLIPW YNTLVFYKEC QIQNHNHTLL LDAWIESELN
     ILINKVNIDM SEYKLNNILN YALDFIENLN NWYIRINRKE LKNNGLYLKT IIKRFSIVMS
     AFVPFFSDYS YQIVCSDKIS VHLEMIPEYK SINSDFNFIK CIIDGIRHLR EKYKLKLKKT
     LKEVIIVLDN ININKNLFEK YEAVIKSECN TLDIILNDIN DYSISTIIKP NFKELNKNKN
     VIKEKLDIIS KLTVNDIENI KNGTHDININ ELLIETYIKD IEYSGVFITL ELF