ID   SYIM_CHICK              Reviewed;        1000 AA.
AC   Q5ZKA2;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Isoleucine--tRNA ligase, mitochondrial;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
DE   Flags: Precursor;
GN   Name=IARS2; ORFNames=RCJMB04_12b19;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ720182; CAG31841.1; -; mRNA.
DR   RefSeq; NP_001006397.1; NM_001006397.1.
DR   AlphaFoldDB; Q5ZKA2; -.
DR   SMR; Q5ZKA2; -.
DR   STRING; 9031.ENSGALP00000015565; -.
DR   PaxDb; Q5ZKA2; -.
DR   PRIDE; Q5ZKA2; -.
DR   GeneID; 421346; -.
DR   KEGG; gga:421346; -.
DR   CTD; 55699; -.
DR   VEuPathDB; HostDB:geneid_421346; -.
DR   eggNOG; KOG0433; Eukaryota.
DR   InParanoid; Q5ZKA2; -.
DR   OrthoDB; 59501at2759; -.
DR   PhylomeDB; Q5ZKA2; -.
DR   PRO; PR:Q5ZKA2; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR   GO; GO:0032543; P:mitochondrial translation; IBA:GO_Central.
DR   CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033708; Anticodon_Ile_BEm.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00392; ileS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..1000
FT                   /note="Isoleucine--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000233338"
FT   MOTIF           102..112
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           649..653
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         652
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1000 AA;  112007 MW;  4EC0778C2FFEB7ED CRC64;
     MLGAWRAAPR LRLRARFGVA SVWARSAASE ANSRRPAEES RQDSQYRDTV LLPHSRFAAQ
     LPGRLQPDTE LETQQKSGFL ELYSWQRQRK AKQEFCLHDG PPYANGDPHV GHALNKILKD
     ITNRFHMMRG YKVHYVPGWD CHGLPIELKA LSEVKGAENL SPVEIRQKAK EFAERAIEKQ
     KAAFIRWGIM ADWANCYRTF DPKYEANQLR VFHKMYDKGF IYQDYKPVFW SPSAKTALAE
     AELEYNEQHV SRSVYMKFPL LKSPPKLASV IDGSSPASVL VWTTQPWTVP ANQAVCYMPD
     AEYSVVKCAT TGEHLILAAD RVESTAAVLD TQFEVISTCK GVDLADGSCA HPTIAGRVSP
     LLPANHVTMT KGTGLVHTAP AHGMEDYNVA SHHQLPTECL VDESGHFTEA AGPELKNKNV
     LEEGNEAVIK MLQAAGSLLK EEKYVHSYPY DWRTKKPMII RASKQWFVNT ANVKATAQEA
     LKKVKIIPTS AVNRMLEMLD RRTFWCISRQ RCWGVPIPVF YQKDTGESLI NSETIADVIK
     IVEQQGTDAW WTLPIEQLLS KEAVAKAGGH NVLDYVKGQD VLDIWFDSGT SWAHVLEGAE
     QRADVYLEGK DQLGGWFQSS LLTSVATRKK APYKTLVVHG FTLGEKGEKM SKSIGNVVDP
     DVVINGGDDH TKDPPYGADV LRWWVAESNV FTEVLIGPVV LNAARDDINK LRNTLRFMLG
     NMAGFNPETD SIPPSEMYIV DQYMLHLLQD YGSKVTEAYK EYDYSKVVRL LQAFCSRNLS
     NFYFSIIKDR LYCEEEKDPK RRSCQTVLAE ALDVVVRSFA PILPHLAEEV FQHLPYKKDS
     EGVFRTGWIN ASSGWKKPGI EEAIEGACAM RDSFLGSISG KNALEYEVII VIEPGLLFEL
     MEALQAEESS RVSQLNEIMM ASQTTLLSEI PKETPADANI VKGNFLINLE GGDIREESSY
     QVIALPTTKA KCPRCRRYTS DSSSTPCPRC LKVIAGKGST