ID   SYIM_CIOIN              Reviewed;         413 AA.
AC   Q94425;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Probable isoleucine--tRNA ligase, mitochondrial;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
DE   Flags: Fragment;
OS   Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC   Cionidae; Ciona.
OX   NCBI_TaxID=7719;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Bird A.P., Clark V., Jones S.J.M., Leitgeb S., Lennard N., Tweedie S.;
RL   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; Z80904; CAB02584.1; -; Genomic_DNA.
DR   PIR; T31663; T31663.
DR   AlphaFoldDB; Q94425; -.
DR   SMR; Q94425; -.
DR   STRING; 7719.XP_002131649.1; -.
DR   eggNOG; KOG0433; Eukaryota.
DR   Proteomes; UP000008144; Unassembled WGS sequence.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR   GO; GO:0032543; P:mitochondrial translation; IBA:GO_Central.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF50677; SSF50677; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           <1..413
FT                   /note="Probable isoleucine--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000098600"
FT   MOTIF           298..302
FT                   /note="'KMSKS' region"
FT   BINDING         301
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   413 AA;  46895 MW;  69B098FD71C100CE CRC64;
     GMICRHPIYL ERTVRMIGSD IANTSKGTGV VHIAPAHGMD DFGIGQKHNL STESSVNSDG
     EFMANIAPNL EGKFIFTSGN EAVVEMLKEE MNLFHEHDYK HSYPYDWRTK QPIFVHTSNQ
     WFVDTAMLAN KAEAAISGIT TYPDNGISSM LNRLQLRSYW CISRQRAWGL PIPVFYHKES
     GELLLNESTI QHLVSLVLKH GADIWWEGTE LLPTQYNADE YERGRDILDI WFDSGVSWDC
     VLRDSKNRTT QADFYIEGKD QYGGWFQSSL LTSVGLQGVA PYKNLMVHGF VLDEKGQKMS
     KSIGNVVDPM VLTNGGKNPQ RDPAYGADAL RWWVAESNVH QDVHISTTII KSAAESVQKI
     RNSVRFMLGN LNEFDKMKIV PTPAMLILDL RYRFEDKKLW YVTSMNYPLN LSV