SYIM_YEAST
ID SYIM_YEAST Reviewed; 1002 AA.
AC P48526; D6W3X3;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Isoleucine--tRNA ligase, mitochondrial;
DE EC=6.1.1.5;
DE AltName: Full=Isoleucyl-tRNA synthetase;
DE Short=IleRS;
GN Name=ISM1; OrderedLocusNames=YPL040C; ORFNames=P7102.10;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 24657 / D273-10B;
RA Tzagoloff A.A., Shtanko A.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- MISCELLANEOUS: Present with 1270 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; L38957; AAA64890.1; -; Genomic_DNA.
DR EMBL; U44030; AAB68180.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11389.1; -; Genomic_DNA.
DR PIR; S62035; S62035.
DR RefSeq; NP_015285.1; NM_001183854.1.
DR AlphaFoldDB; P48526; -.
DR SMR; P48526; -.
DR BioGRID; 36139; 95.
DR DIP; DIP-4034N; -.
DR IntAct; P48526; 3.
DR MINT; P48526; -.
DR STRING; 4932.YPL040C; -.
DR MaxQB; P48526; -.
DR PaxDb; P48526; -.
DR PRIDE; P48526; -.
DR EnsemblFungi; YPL040C_mRNA; YPL040C; YPL040C.
DR GeneID; 856067; -.
DR KEGG; sce:YPL040C; -.
DR SGD; S000005961; ISM1.
DR VEuPathDB; FungiDB:YPL040C; -.
DR eggNOG; KOG0433; Eukaryota.
DR GeneTree; ENSGT00550000074910; -.
DR HOGENOM; CLU_001493_7_2_1; -.
DR InParanoid; P48526; -.
DR OMA; PVYWGTE; -.
DR BioCyc; YEAST:G3O-33954-MON; -.
DR PRO; PR:P48526; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P48526; protein.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IMP:SGD.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; ISA:SGD.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; ISA:SGD.
DR GO; GO:0070152; P:mitochondrial isoleucyl-tRNA aminoacylation; IC:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00392; ileS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..1002
FT /note="Isoleucine--tRNA ligase, mitochondrial"
FT /id="PRO_0000098605"
FT MOTIF 94..104
FT /note="'HIGH' region"
FT MOTIF 668..672
FT /note="'KMSKS' region"
FT BINDING 671
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 437
FT /note="L -> P (in Ref. 1; AAA64890)"
FT /evidence="ECO:0000305"
FT CONFLICT 672
FT /note="S -> L (in Ref. 1; AAA64890)"
FT /evidence="ECO:0000305"
FT CONFLICT 806
FT /note="D -> G (in Ref. 1; AAA64890)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1002 AA; 115794 MW; 2785B9DED636529E CRC64;
MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI PKSSQLVYKE
QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL HLGHALNKIL KDIINRYQLS
QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ IESISPLKIR SMALKHAQKA IKRQRETFQH
FAILTDWETP YLTMDKDYEI NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN
ENHKSIAAYV KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY
SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD KVGRPLLHGA
HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG RYQLNELPQS VRSIVRDEGD
LTKGRQVLDA ETAKIILCKL SDLNLLYKSH EYTHSYPYDW RSKKPVIIRA TPQWFADLHD
VKNLALESIS RVKFCPKRGY SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN
SEILAHAIEK IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS
VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA PYEEVITHGF
TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR YLIAQSNFTT DIVAGPTVMK
HVGEALKKVR LTFRYLLSNL QKSQDFNLLP IEQLRRVDQY TLYKINELLE TTREHYQKYN
FSKVLITLQY HLNNELSAFY FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL
PVMVQEVWKY IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK
RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN SLPTIELGSG
INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL MS
