ID   BLAI_MYCTO              Reviewed;         138 AA.
AC   P9WMJ4; L0T7V6; P95163; Q7D7W9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Transcriptional regulator BlaI;
GN   Name=blaI; OrderedLocusNames=MT1894;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Transcription regulator that regulates genes involved in
CC       antibiotic transport, detoxification and cell wall function. Also
CC       regulates its own transcription. Binds DNA as a dimer (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Upon exposure to beta-lactams, proteolytic cleavage at a single
CC       site may impair dimerization and abolish repressor activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BlaI transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK46165.1; -; Genomic_DNA.
DR   PIR; F70664; F70664.
DR   RefSeq; WP_003409301.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WMJ4; -.
DR   SMR; P9WMJ4; -.
DR   EnsemblBacteria; AAK46165; AAK46165; MT1894.
DR   GeneID; 45425819; -.
DR   KEGG; mtc:MT1894; -.
DR   PATRIC; fig|83331.31.peg.2038; -.
DR   HOGENOM; CLU_119090_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005650; BlaI_family.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR34134; PTHR34134; 1.
DR   Pfam; PF03965; Penicillinase_R; 1.
DR   PIRSF; PIRSF019455; CopR_AtkY; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..138
FT                   /note="Transcriptional regulator BlaI"
FT                   /id="PRO_0000427293"
FT   DNA_BIND        6..71
FT                   /note="H-T-H motif"
FT   REGION          74..138
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   SITE            102..103
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   138 AA;  15211 MW;  86F073B72F3CBD98 CRC64;
     MAKLTRLGDL ERAVMDHLWS RTEPQTVRQV HEALSARRDL AYTTVMTVLQ RLAKKNLVLQ
     IRDDRAHRYA PVHGRDELVA GLMVDALAQA EDSGSRQAAL VHFVERVGAD EADALRRALA
     ELEAGHGNRP PAGAATET