ID   BLC01_BRELC             Reviewed;         198 AA.
AC   A0A3B7TNW2;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Crinkler effector protein BLC01 {ECO:0000303|PubMed:30251420};
DE   Flags: Precursor;
GN   Name=BLC01 {ECO:0000303|PubMed:30251420};
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=Race Bl:24;
RX   PubMed=30251420; DOI=10.1111/mpp.12751;
RA   Pelgrom A.J.E., Eikelhof J., Elberse J., Meisrimler C.N., Raedts R.,
RA   Klein J., Van den Ackerveken G.;
RT   "Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola
RT   LS102 requires two unlinked loci.";
RL   Mol. Plant Pathol. 20:240-253(2019).
CC   -!- FUNCTION: Secreted effector that elicits necrosis in host plants, a
CC       characteristic of plant innate immunity. {ECO:0000305|PubMed:30251420}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:30251420}. Host cell
CC       {ECO:0000305|PubMed:30251420}.
CC   -!- DOMAIN: The CRN proteins have modular architectures that include a
CC       signal peptide, a conserved N-terminus, and highly diverse C-terminal
CC       domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif,
CC       which is followed by the conserved DWL domain. A highly conserved
CC       HVLVXXP motif marks the end of the CRN N-terminal domains and forms a
CC       junction where diverse C-terminal effector domains are fused. The
CC       conserved CRN N-terminus mediates the translocation into the plant host
CC       cells. {ECO:0000305|PubMed:30251420}.
CC   -!- SIMILARITY: Belongs to the Crinkler effector family. {ECO:0000305}.
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DR   EMBL; MG686573; AYE92117.1; -; mRNA.
DR   AlphaFoldDB; A0A3B7TNW2; -.
DR   VEuPathDB; FungiDB:CCR75_001066; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043657; C:host cell; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045379; Crinkler_N.
DR   Pfam; PF20147; Crinkler; 1.
PE   2: Evidence at transcript level;
KW   Secreted; Signal; Virulence.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..198
FT                   /note="Crinkler effector protein BLC01"
FT                   /id="PRO_5017536872"
FT   REGION          16..55
FT                   /note="LQLFLAK domain"
FT                   /evidence="ECO:0000305|PubMed:30251420"
FT   REGION          56..113
FT                   /note="DWL domain"
FT                   /evidence="ECO:0000305|PubMed:30251420"
FT   MOTIF           114..119
FT                   /note="HVLVXXP motif"
FT                   /evidence="ECO:0000305|PubMed:30251420"
SQ   SEQUENCE   198 AA;  22069 MW;  6D3F4ED846AF82AB CRC64;
     MMVKLICAIV DIAGAAFPID IDTNELVGDF KKVIKAENSR TIACDANDLR LFLAKTDGRW
     LTEFEVQNGV ADISVFEELD VVGAPLNMIG LSEETVSSVA ITKELVKAKK TPLHVLVVPS
     EPVQLQRKLW LVTGTVVNAL GSKGIRRHLH LMASLHIGFY DPTRRVDNKN VAFWYEAKNL
     CFHVLFKSST CFFYVSPF