BLC97_ECOLX
ID BLC97_ECOLX Reviewed; 291 AA.
AC E1ANH6;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Beta-lactamase CTX-M-97;
DE EC=3.5.2.6;
DE Flags: Precursor;
GN Name=bla;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE FUNCTION.
RC STRAIN=B275;
RX PubMed=23117265; DOI=10.1007/s10096-012-1765-9;
RA Karfunkel D., Carmeli Y., Chmelnitsky I., Kotlovsky T., Navon-Venezia S.;
RT "The emergence and dissemination of CTX-M-producing Escherichia coli
RT sequence type 131 causing community-onset bacteremia in Israel.";
RL Eur. J. Clin. Microbiol. Infect. Dis. 32:513-521(2013).
CC -!- FUNCTION: Is probably capable of hydrolyzing cephalosporins such as
CC ceftriaxone and ceftazidime, thus conferring resistance to these
CC antibiotics.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10101};
CC -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HM776707; ADM08178.1; -; Genomic_DNA.
DR RefSeq; WP_063860093.1; NG_049051.1.
DR PDB; 5A90; Neutron; 1.70 A; A=30-291.
DR PDB; 5A91; X-ray; 1.20 A; A=30-291.
DR PDB; 5A92; X-ray; 1.05 A; A=30-291.
DR PDB; 5A93; Other; 1.60 A; A=30-291.
DR PDB; 5G18; X-ray; 1.10 A; A=30-291.
DR PDBsum; 5A90; -.
DR PDBsum; 5A91; -.
DR PDBsum; 5A92; -.
DR PDBsum; 5A93; -.
DR PDBsum; 5G18; -.
DR AlphaFoldDB; E1ANH6; -.
DR SMR; E1ANH6; -.
DR KEGG; ag:ADM08178; -.
DR GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 3.40.710.10; -; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR045155; Beta-lactam_cat.
DR InterPro; IPR000871; Beta-lactam_class-A.
DR InterPro; IPR023650; Beta-lactam_class-A_AS.
DR PANTHER; PTHR35333; PTHR35333; 1.
DR Pfam; PF13354; Beta-lactamase2; 1.
DR PRINTS; PR00118; BLACTAMASEA.
DR SUPFAM; SSF56601; SSF56601; 1.
DR PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Hydrolase; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..291
FT /note="Beta-lactamase CTX-M-97"
FT /id="PRO_0000420868"
FT ACT_SITE 73
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT BINDING 237..239
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT HELIX 33..44
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 46..54
FT /evidence="ECO:0007829|PDB:5A92"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 60..64
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 72..75
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 76..87
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 112..115
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 118..121
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 122..132
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 135..145
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 148..157
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 171..173
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 186..198
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 204..215
FT /evidence="ECO:0007829|PDB:5A92"
FT TURN 221..223
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 224..227
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 232..240
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 246..253
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 255..257
FT /evidence="ECO:0007829|PDB:5A92"
FT STRAND 260..267
FT /evidence="ECO:0007829|PDB:5A92"
FT HELIX 277..288
FT /evidence="ECO:0007829|PDB:5A92"
SQ SEQUENCE 291 AA; 31279 MW; 8E91A7B8C810A2D1 CRC64;
MMTQSIGRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV ALINTADNSQ
ILYRADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI KKSDLVNYNP IAEKHVNGTM
TLAELGAAAL QYSDNTAMNK LIAHLGGPDK VTAFARSLGD ETFRLDRTEP TLNTAIPGDP
RDTTTPLAMA QTLKNLTLGK ALAETQRAQL VTWLKGNTTG SASIRAGLPK SWVVGDKTGS
GDYGTTNDIA VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F
