ID   SYI_TETTH               Reviewed;        1081 AA.
AC   P36422;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Isoleucine--tRNA ligase;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IleRS;
GN   Name=ILSA; Synonyms=CUPC;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1371507; DOI=10.1016/s0021-9258(18)42874-8;
RA   Csank C., Martindale D.W.;
RT   "Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena
RT   thermophila. DNA sequence, gene regulation, and leucine zipper motifs.";
RL   J. Biol. Chem. 267:4592-4599(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; M30942; AAA30122.1; -; Genomic_DNA.
DR   PIR; A42399; A42399.
DR   AlphaFoldDB; P36422; -.
DR   SMR; P36422; -.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033709; Anticodon_Ile_ABEc.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR42780; PTHR42780; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00392; ileS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           1..1081
FT                   /note="Isoleucine--tRNA ligase"
FT                   /id="PRO_0000098602"
FT   MOTIF           53..63
FT                   /note="'HIGH' region"
FT   MOTIF           607..611
FT                   /note="'KMSKS' region"
FT   BINDING         610
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1081 AA;  124851 MW;  D4D72616AFB8C795 CRC64;
     MASKTTFKDL QEIPDFPKEE ENILKFWDEI NAFKQQLEKT KDCPPFTFYD GPPFATGLPH
     YGNLLAGTIK DVVCRYASQN GKYVERRFGW DCHGLPVEYE IDKKLGITNR QEVLKMGVDK
     YNAECRSIVM RYAQEWRSIV NRFGRWVDFD NDYKTLDLKF MESVWWVFKQ MFDKGLVYRG
     CKVMPYSNGC ATVLSNFETQ QNYKEVDDPS LFIAFKTAED PKTKFIAWTT TPWTLPSNLA
     LVINKDFDYV KVLDAKTQEH YILAECRLPE LYKKDKDGYK ILEKFKGSEL VGREYEPLFP
     YFLSRKQDGC FRILAGDFVT ADAGTGIVHC APGFGDDDYK VSVANNIIKP DDPPVPVDEN
     GHFTNVVSDF AGVYIKEADK LIRKNLKERG LLLVDSSFKH NYPFCWRSDT PLIYKAVHCW
     FIKVTALKDD LLANNKKAYW VPKFAQEGRF NNWLQNVSDW CFSRSRFWGN PIPIWVSEDF
     EEVVCIGSVE ELKKLTGATE ITDLHKDFID HLTIPSQKGK GVLRRIDEVF DCWFESGSMP
     YGQQHYPFSM NEEEFSKRFP ADFIGEGIDQ TRGWFYTLNV ISTALRNSNP YKNLIVNGIV
     LAADGKKMSK SKKNYDDPLL IASKYSVDAI RLYMINSPLV RAEEMSFKSE GVFAVKKDIF
     LPWYNAYKFL IQSITRWELA TGKDYMFNEQ LSVDTTKLTN PTDRWIIISC QNLINYVRIE
     MEKYHLYNVV PRLIHFLENL TNWYIRLNRN RLKGDYGLEE QETSLNVLFN VILNSTILMS
     PLVPFITESF YQNLRKVIPK GSSYLEESIH FLRIPTPKQE LLDEKIERNF ERMQNIINFA
     RTLREKRKVS LKQPIMSLTV INQDQEFHDS LKDYIQYIED EINTPSILHE INTANYVDLK
     AIPNHKLLGQ KLGKEYNKDL KAAAGNLSAK DIETLKTTGS IDLVGKKLLL EDFTITQNYK
     KEYSSGDLEL GGEGEVILLL NLAQDEKLKS KGLVREFTSN VQKTKKKTGL KVDDNIVIYY
     DVTKAPKLNA AIQSDLEAVQ KVLKKPLVPL SEKNAALKIV ENSLLTFQVD EESVTIEIAY
     A