ID   SYI_THEFY               Reviewed;        1060 AA.
AC   Q47QV9;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Isoleucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_02003};
DE            EC=6.1.1.5 {ECO:0000255|HAMAP-Rule:MF_02003};
DE   AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_02003};
DE            Short=IleRS {ECO:0000255|HAMAP-Rule:MF_02003};
GN   Name=ileS {ECO:0000255|HAMAP-Rule:MF_02003}; OrderedLocusNames=Tfu_1120;
OS   Thermobifida fusca (strain YX).
OC   Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
OC   Thermobifida.
OX   NCBI_TaxID=269800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YX;
RX   PubMed=17209016; DOI=10.1128/jb.01899-06;
RA   Lykidis A., Mavromatis K., Ivanova N., Anderson I., Land M., DiBartolo G.,
RA   Martinez M., Lapidus A., Lucas S., Copeland A., Richardson P., Wilson D.B.,
RA   Kyrpides N.;
RT   "Genome sequence and analysis of the soil cellulolytic actinomycete
RT   Thermobifida fusca YX.";
RL   J. Bacteriol. 189:2477-2486(2007).
CC   -!- FUNCTION: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS
CC       can inadvertently accommodate and process structurally similar amino
CC       acids such as valine, to avoid such errors it has two additional
CC       distinct tRNA(Ile)-dependent editing activities. One activity is
CC       designated as 'pretransfer' editing and involves the hydrolysis of
CC       activated Val-AMP. The other activity is designated 'posttransfer'
CC       editing and involves deacylation of mischarged Val-tRNA(Ile).
CC       {ECO:0000255|HAMAP-Rule:MF_02003}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_02003};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02003};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_02003}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02003}.
CC   -!- DOMAIN: IleRS has two distinct active sites: one for aminoacylation and
CC       one for editing. The misactivated valine is translocated from the
CC       active site to the editing site, which sterically excludes the
CC       correctly activated isoleucine. The single editing site contains two
CC       valyl binding pockets, one specific for each substrate (Val-AMP or Val-
CC       tRNA(Ile)). {ECO:0000255|HAMAP-Rule:MF_02003}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       IleS type 2 subfamily. {ECO:0000255|HAMAP-Rule:MF_02003}.
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DR   EMBL; CP000088; AAZ55158.1; -; Genomic_DNA.
DR   RefSeq; WP_011291567.1; NC_007333.1.
DR   AlphaFoldDB; Q47QV9; -.
DR   SMR; Q47QV9; -.
DR   STRING; 269800.Tfu_1120; -.
DR   PRIDE; Q47QV9; -.
DR   EnsemblBacteria; AAZ55158; AAZ55158; Tfu_1120.
DR   KEGG; tfu:Tfu_1120; -.
DR   eggNOG; COG0060; Bacteria.
DR   HOGENOM; CLU_001493_1_1_11; -.
DR   OMA; PSWYIRT; -.
DR   OrthoDB; 32262at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033709; Anticodon_Ile_ABEc.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR42780; PTHR42780; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00392; ileS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase; Metal-binding;
KW   Nucleotide-binding; Protein biosynthesis; Zinc.
FT   CHAIN           1..1060
FT                   /note="Isoleucine--tRNA ligase"
FT                   /id="PRO_0000098565"
FT   MOTIF           55..65
FT                   /note="'HIGH' region"
FT   MOTIF           608..612
FT                   /note="'KMSKS' region"
FT   BINDING         611
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02003"
SQ   SEQUENCE   1060 AA;  118881 MW;  3DA876E2028CE987 CRC64;
     MSKESTRPFP TLPAQIDLPA MEHEVLRRWE EHKIFERSLE QTQGGPTWVF YEGPPTANGT
     PGVHHVEARV FKDVFPRFKT MKGFYVERKA GWDCHGLPVE VAVEKELGIS GKKDIEAFGI
     AEFNARCRES VLRNVDAFTE MTRRMGYWVN MDEAYRTMDR EYVESVWWAI KQIWDKGLLV
     QDYRISPYCP RCGTTLSDHE LAQGYETVTD PSVYVRFPLT SGPLAGQAAL LVWTTTPWTL
     VSNTAVAVHP EVDYVVATDG SEQLVVAEPL VGAALGDGWT LTGQRFKGAE LERWTYQRPF
     ELVEFDSPAH FVVLGDYVTV EDGTGLVHQA PAFGADDMQV CRAYGLPVVN PVRNDGTFEE
     HLDLVGDEFF KTADAALVAD LKDRGLLFKH LDYEHSYPHC WRCHTPLMYY AVPSWYIKTT
     AIKDQLLAEN AKTNWVPANV KDGRYGEWLR NNVDWALSRS RYWGTPLPIW EFPDGRRICV
     GSLKELSELS GQDLSDLDPH RPYVDDIVIP DPDADPSLPL EQRVARRVPE VIDVWFDSGA
     MPFAQWGAPH RNQEKFEANF PAQYICEAID QTRGWFYSLM AVSTLVFGRS SYENVVCLGH
     ILAEDGRKMS KHLGNILEPI PVMDRHGADA LRWFMAASGS PWMPRRVGHT VLEEIVRKVL
     LTYYNSASFF TLYAGAGDGW SHEQLADAPA PQDRPLLDRW ILSELHSLIK TVDDALERFD
     TALAGRALTT FIDDLSNWYV RRSRRRFWAG AGTPEGAAAF ATLFECLETL TLLMAPIVPF
     ITDHVWQALR RPDAPESVHL ASWPKADESL IDPALSEQMA LVRRLVELGR AARVDSGQRV
     RQPLARALVG APGFAELPEQ LRAQIAEELN VVQLDPLSVV GGDLVDYSVK PNFRALGKRF
     GKTTPRVAQA IREADAKTLV ERLRADNAAT VDVDGEQVVL SADEVVVTEQ PREGWTVASE
     AGETVALDLE LTPELRRAGV AREVIRLVQD ARKSSGLNIS DRIHLWWSAT DEMTAQAMAE
     HAEAISSEVL AVSFTEGTGD ADAYEVVSEE FGITLRLRKA