ID   BLE_KLEPN               Reviewed;         126 AA.
AC   P13081;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Bleomycin resistance protein;
DE            Short=BRP;
DE   AltName: Full=CL990 resistance protein;
GN   Name=ble;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3889831; DOI=10.1093/nar/13.1.195;
RA   Mazodier P., Cossart P., Giraud E., Gasser F.;
RT   "Completion of the nucleotide sequence of the central region of Tn5
RT   confirms the presence of three resistance genes.";
RL   Nucleic Acids Res. 13:195-205(1985).
CC   -!- FUNCTION: Binding protein with a strong affinity to the bleomycin
CC       family of antibiotics. Binds to CL990; an antimitotic-antibiotic
CC       compound.
CC   -!- MISCELLANEOUS: This enzyme is encoded by the kanamycin and neomycin
CC       resistance transposon Tn5.
CC   -!- SIMILARITY: Belongs to the bleomycin resistance protein family.
CC       {ECO:0000305}.
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DR   EMBL; U00004; AAA73391.1; -; Unassigned_DNA.
DR   EMBL; X01702; CAA25853.1; -; Genomic_DNA.
DR   RefSeq; WP_000131935.1; NZ_VKSL01000036.1.
DR   RefSeq; YP_788127.1; NC_008460.1.
DR   PDB; 1ECS; X-ray; 1.70 A; A/B=1-126.
DR   PDB; 1EWJ; X-ray; 2.50 A; A/B/C/D/E/F/G/H=1-126.
DR   PDB; 1MH6; NMR; -; A/B=2-126.
DR   PDB; 1NIQ; NMR; -; B/C=1-126.
DR   PDBsum; 1ECS; -.
DR   PDBsum; 1EWJ; -.
DR   PDBsum; 1MH6; -.
DR   PDBsum; 1NIQ; -.
DR   AlphaFoldDB; P13081; -.
DR   SMR; P13081; -.
DR   EvolutionaryTrace; P13081; -.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.180.10; -; 1.
DR   InterPro; IPR000335; Bleomycin-R.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR037523; VOC.
DR   PRINTS; PR00311; BLEOMYCINRST.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   PROSITE; PS51819; VOC; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Transposable element.
FT   CHAIN           1..126
FT                   /note="Bleomycin resistance protein"
FT                   /id="PRO_0000068561"
FT   DOMAIN          1..119
FT                   /note="VOC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   STRAND          4..12
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   HELIX           14..22
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   TURN            23..25
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          27..31
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          33..40
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          43..49
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   HELIX           55..57
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          61..67
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   HELIX           69..78
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          85..96
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          100..107
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   STRAND          113..118
FT                   /evidence="ECO:0007829|PDB:1ECS"
FT   HELIX           121..124
FT                   /evidence="ECO:0007829|PDB:1MH6"
SQ   SEQUENCE   126 AA;  14074 MW;  D0FDB083AE3D2334 CRC64;
     MTDQATPNLP SRDFDSTAAF YERLGFGIVF RDAGWMILQR GDLMLEFFAH PGLDPLASWF
     SCCLRLDDLA EFYRQCKSVG IQETSSGYPR IHAPELQEWG GTMAALVDPD GTLLRLIQNE
     LLAGIS