ID   BLE_STAAN               Reviewed;         132 AA.
AC   Q7A8D1; P13014; P22491; Q79ER2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Bleomycin resistance protein;
DE            Short=BRP;
DE   AltName: Full=Bleomycin-binding protein;
GN   Name=ble; Synonyms=bleO; OrderedLocusNames=SA0032;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10348769; DOI=10.1128/aac.43.6.1449;
RA   Ito T., Katayama Y., Hiramatsu K.;
RT   "Cloning and nucleotide sequence determination of the entire mec DNA of
RT   pre-methicillin-resistant Staphylococcus aureus N315.";
RL   Antimicrob. Agents Chemother. 43:1449-1458(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RA   Stenz L.;
RT   "Shotgun proteomic analysis of total protein extract of S. aureus S30
RT   versus N315.";
RL   Submitted (NOV-2005) to UniProtKB.
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- FUNCTION: Binding protein with a strong affinity to the bleomycin
CC       family of antibiotics, which confers resistance to these antibiotics by
CC       preventing the bleomycin-induced DNA breakage. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bleomycin resistance protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA82230.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB41250.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; D86934; BAA82230.2; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000018; BAB41250.1; ALT_INIT; Genomic_DNA.
DR   PIR; A89762; A89762.
DR   PIR; T44128; T44128.
DR   AlphaFoldDB; Q7A8D1; -.
DR   SMR; Q7A8D1; -.
DR   EnsemblBacteria; BAB41250; BAB41250; BAB41250.
DR   KEGG; sau:SA0032; -.
DR   HOGENOM; CLU_046006_15_5_9; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd08349; BLMA_like; 1.
DR   Gene3D; 3.10.180.10; -; 1.
DR   InterPro; IPR000335; Bleomycin-R.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR037523; VOC.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   PRINTS; PR00311; BLEOMYCINRST.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   PROSITE; PS51819; VOC; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance.
FT   CHAIN           1..132
FT                   /note="Bleomycin resistance protein"
FT                   /id="PRO_0000300632"
FT   DOMAIN          1..129
FT                   /note="VOC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
SQ   SEQUENCE   132 AA;  14922 MW;  2D7A1E2EF7187CBA CRC64;
     MLQSIPALPV GDIKKSIGFY CDKLGFTLVH HEDGFAVLMC NEVRIHLWEA SDEGWRSRSN
     DSPVCTGAES FIAGTASCRI EVEGIDELYQ HIKPLGILHP NTSLKDQWWD ERDFAVIDPD
     NNLISFFQQI KS