ID   BLE_STAAU               Reviewed;         132 AA.
AC   Q7DJ53; P13014; P22491; Q6LDT5; Q79ER2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Bleomycin resistance protein;
DE            Short=BRP;
DE   AltName: Full=Bleomycin-binding protein;
GN   Name=ble; Synonyms=bleO, blmS;
OS   Staphylococcus aureus.
OG   Plasmid pUB110, and Plasmid pSK41.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pUB110;
RX   PubMed=3744038;
RA   Bashkirov V.I., Mil'Shina N.V., Prozorov A.A.;
RT   "Nucleotide sequence and physical map of kanamycin-resistant plasmid pUB110
RT   from Staphylococcus aureus.";
RL   Genetika 22:1081-1092(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pUB110;
RX   PubMed=3010356; DOI=10.1016/0147-619x(86)90046-6;
RA   McKenzie T., Hoshino T., Tanaka T., Sueoka N.;
RT   "The nucleotide sequence of pUB110: some salient features in relation to
RT   replication and its regulation.";
RL   Plasmid 15:93-103(1986).
RN   [3]
RP   SEQUENCE REVISION.
RX   PubMed=3033723; DOI=10.1016/0147-619x(87)90015-1;
RA   McKenzie T., Hoshino T., Tanaka T., Sueoka N.;
RT   "Correction. A revision of the nucleotide sequence and functional map of
RT   pUB110.";
RL   Plasmid 17:83-85(1987).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pUB110;
RX   PubMed=2437604; DOI=10.1016/0147-619x(87)90007-2;
RA   Semon D., Movva N.R., Smith T.F., Alama M.E., Davies J.E.;
RT   "Plasmid-determined bleomycin resistance in Staphylococcus aureus.";
RL   Plasmid 17:46-53(1987).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pSK41;
RX   PubMed=9721269; DOI=10.1128/jb.180.17.4350-4359.1998;
RA   Berg T., Firth N., Apisiridej S., Hettiaratchi A., Leelaporn A.,
RA   Skurray R.A.;
RT   "Complete nucleotide sequence of pSK41: evolution of staphylococcal
RT   conjugative multiresistance plasmids.";
RL   J. Bacteriol. 180:4350-4359(1998).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HUC19;
RX   PubMed=10858352; DOI=10.1128/aac.44.7.1906-1910.2000;
RA   Oliveira D.C., Wu S.W., de Lencastre H.;
RT   "Genetic organization of the downstream region of the mecA element in
RT   methicillin-resistant Staphylococcus aureus isolates carrying different
RT   polymorphisms of this region.";
RL   Antimicrob. Agents Chemother. 44:1906-1910(2000).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MRSA B-26, and MRSA K-1;
RX   PubMed=12111746;
RX   DOI=10.1002/1521-4028(200206)42:3<190::aid-jobm190>3.0.co;2-8;
RA   McElgunn C.J., Bhuiyan M.Z.A., Sugiyama M.;
RT   "Integration analysis of pSK41 in the chromosome of a methicillin-resistant
RT   Staphylococcus aureus K-1.";
RL   J. Basic Microbiol. 42:190-200(2002).
RN   [8]
RP   PROTEIN SEQUENCE OF 1-15, AND FUNCTION.
RC   STRAIN=MRSA B-26;
RX   PubMed=7535252; DOI=10.1016/0014-5793(95)00218-x;
RA   Sugiyama M., Kumagai T., Matsuo H., Bhuiyan M.Z.A., Ueda K., Mochizuki H.,
RA   Nakamura N., Davies J.E.;
RT   "Overproduction of the bleomycin-binding proteins from bleomycin-producing
RT   Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus
RT   in Escherichia coli and their immunological characterisation.";
RL   FEBS Lett. 362:80-84(1995).
CC   -!- FUNCTION: Binding protein with a strong affinity to the bleomycin
CC       family of antibiotics, which confers resistance to these antibiotics by
CC       preventing the bleomycin-induced DNA breakage.
CC       {ECO:0000269|PubMed:7535252}.
CC   -!- SIMILARITY: Belongs to the bleomycin resistance protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA98213.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M37273; AAA98213.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; M19465; AAA88360.1; -; Genomic_DNA.
DR   EMBL; M20270; AAA26671.1; -; Genomic_DNA.
DR   EMBL; AF051917; AAC61949.1; -; Genomic_DNA.
DR   EMBL; AF181950; AAF85648.1; -; Genomic_DNA.
DR   EMBL; AB037419; BAC00981.1; -; Genomic_DNA.
DR   EMBL; AB037420; BAC00987.1; -; Genomic_DNA.
DR   PIR; T44128; T44128.
DR   RefSeq; NP_040432.1; NC_001384.1.
DR   RefSeq; NP_863620.1; NC_005024.1.
DR   RefSeq; WP_001621591.1; NZ_WKGG01000017.1.
DR   RefSeq; YP_002790915.1; NC_012547.1.
DR   RefSeq; YP_006937661.1; NC_013320.1.
DR   AlphaFoldDB; Q7DJ53; -.
DR   SMR; Q7DJ53; -.
DR   PRO; PR:Q7DJ53; -.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd08349; BLMA_like; 1.
DR   Gene3D; 3.10.180.10; -; 1.
DR   InterPro; IPR000335; Bleomycin-R.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR037523; VOC.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   PRINTS; PR00311; BLEOMYCINRST.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   PROSITE; PS51819; VOC; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Direct protein sequencing; Plasmid.
FT   CHAIN           1..132
FT                   /note="Bleomycin resistance protein"
FT                   /id="PRO_0000068562"
FT   DOMAIN          1..129
FT                   /note="VOC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   CONFLICT        21
FT                   /note="C -> W (in Ref. 1; AAA98213)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        24
FT                   /note="L -> V (in Ref. 1; AAA98213)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   132 AA;  14922 MW;  2D7A1E2EF7187CBA CRC64;
     MLQSIPALPV GDIKKSIGFY CDKLGFTLVH HEDGFAVLMC NEVRIHLWEA SDEGWRSRSN
     DSPVCTGAES FIAGTASCRI EVEGIDELYQ HIKPLGILHP NTSLKDQWWD ERDFAVIDPD
     NNLISFFQQI KS