ID   BLE_STRHI               Reviewed;         124 AA.
AC   P17493;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Bleomycin resistance protein;
DE            Short=BRP;
DE   AltName: Full=Phleomycin resistance protein;
GN   Name=ble;
OS   Streptoalloteichus hindustanus.
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Streptoalloteichus.
OX   NCBI_TaxID=2017;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31158 / E465-94;
RX   PubMed=1695734; DOI=10.1093/nar/18.13.4009;
RA   Drocourt D., Calmels T., Reynes J.-P., Baron M., Tiraby G.;
RT   "Cassettes of the Streptoalloteichus hindustanus ble gene for
RT   transformation of lower and higher eukaryotes to phleomycin resistance.";
RL   Nucleic Acids Res. 18:4009-4009(1990).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RC   STRAIN=ATCC 31158 / E465-94;
RX   PubMed=7516875; DOI=10.1002/j.1460-2075.1994.tb06535.x;
RA   Dumas P., Bergdoll M., Cagnon C., Masson J.-M.;
RT   "Crystal structure and site-directed mutagenesis of a bleomycin resistance
RT   protein and their significance for drug sequestering.";
RL   EMBO J. 13:2483-2492(1994).
CC   -!- FUNCTION: Binding protein with a strong affinity to the bleomycin
CC       family of antibiotics.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the bleomycin resistance protein family.
CC       {ECO:0000305}.
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DR   EMBL; X52869; CAA37050.1; -; Genomic_DNA.
DR   PIR; S10304; S10304.
DR   RefSeq; WP_063842998.1; NZ_FQVN01000005.1.
DR   PDB; 1BYL; X-ray; 2.30 A; A=1-124.
DR   PDB; 1XRK; X-ray; 1.50 A; A/B=1-124.
DR   PDB; 2ZHP; X-ray; 1.60 A; A/B=1-124.
DR   PDBsum; 1BYL; -.
DR   PDBsum; 1XRK; -.
DR   PDBsum; 2ZHP; -.
DR   AlphaFoldDB; P17493; -.
DR   BMRB; P17493; -.
DR   SMR; P17493; -.
DR   EvolutionaryTrace; P17493; -.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd08349; BLMA_like; 1.
DR   Gene3D; 3.10.180.10; -; 1.
DR   InterPro; IPR000335; Bleomycin-R.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR037523; VOC.
DR   Pfam; PF19581; Glyoxalase_7; 1.
DR   PRINTS; PR00311; BLEOMYCINRST.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   PROSITE; PS51819; VOC; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance.
FT   CHAIN           1..124
FT                   /note="Bleomycin resistance protein"
FT                   /id="PRO_0000068563"
FT   DOMAIN          3..121
FT                   /note="VOC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   STRAND          3..14
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   HELIX           16..25
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          30..34
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          36..43
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          46..52
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   TURN            56..58
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   HELIX           59..61
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          63..69
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   HELIX           71..78
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   TURN            79..81
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          82..84
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   TURN            86..88
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          90..94
FT                   /evidence="ECO:0007829|PDB:1BYL"
FT   STRAND          98..100
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          103..109
FT                   /evidence="ECO:0007829|PDB:1XRK"
FT   STRAND          115..120
FT                   /evidence="ECO:0007829|PDB:1XRK"
SQ   SEQUENCE   124 AA;  13796 MW;  13CCB17F1CC14887 CRC64;
     MAKLTSAVPV LTARDVAGAV EFWTDRLGFS RDFVEDDFAG VVRDDVTLFI SAVQDQVVPD
     NTLAWVWVRG LDELYAEWSE VVSTNFRDAS GPAMTEIGEQ PWGREFALRD PAGNCVHFVA
     EEQD