ID   SYKC_NOSCE              Reviewed;         514 AA.
AC   C4V8R9;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Probable lysine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.6;
DE   AltName: Full=Lysyl-tRNA synthetase;
DE            Short=LysRS;
GN   ORFNames=NCER_100909;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ACOL01000065; EEQ82379.1; -; Genomic_DNA.
DR   RefSeq; XP_002996050.1; XM_002996004.1.
DR   AlphaFoldDB; C4V8R9; -.
DR   SMR; C4V8R9; -.
DR   STRING; 578460.C4V8R9; -.
DR   EnsemblFungi; EEQ82379; EEQ82379; NCER_100909.
DR   KEGG; nce:NCER_100909; -.
DR   VEuPathDB; MicrosporidiaDB:NCER_100909; -.
DR   HOGENOM; CLU_008255_6_0_1; -.
DR   InParanoid; C4V8R9; -.
DR   OMA; EIFGEKC; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00775; LysRS_core; 1.
DR   CDD; cd04322; LysRS_N; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   HAMAP; MF_00252; Lys_tRNA_synth_class2; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR002313; Lys-tRNA-ligase_II.
DR   InterPro; IPR034762; Lys-tRNA-ligase_II_bac/euk.
DR   InterPro; IPR044136; Lys-tRNA-ligase_II_N.
DR   InterPro; IPR018149; Lys-tRNA-synth_II_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PIRSF; PIRSF039101; LysRS2; 1.
DR   PRINTS; PR00982; TRNASYNTHLYS.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00499; lysS_bact; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..514
FT                   /note="Probable lysine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388396"
SQ   SEQUENCE   514 AA;  59437 MW;  88778AA701931658 CRC64;
     MSTKNNSNVP EISDEEFYKN RLLTVTEQLK DNVQVYPHKY EVNYRFKDIF TFKDASEEDL
     KKVKVQSAGR ILNFRIHARY SFFQVMSEDF TIQLVVDAQV LENKDIISNI KRGDIVGFSG
     VLGRTKTKEF SVFIKELSIL TPCLRTIPTD YYGLKDPEII YRKRYLDLLI NKESKNRFIQ
     RTNIIKFIRK YLDDKDFVEV ETPLLNIIPT GAAAKPFTTH HNELKMNLFL RIAPELYLKK
     LVIGGMDRVY EIGKLFRNEG IDLTHNPEFT ACEFYMAYAD YNDMMNMAEE MLNGMCKYLH
     GSEKIVYAPN KREKEVKPVE INFARPFARF HMLEELSKVV GIKLDGLNIN SDETLDLLIE
     TCDKYEIKVE QPKTLTRVLD KLVGHFIEPK CINPSFIIGY PLVTSPLAKN HRSEAGMVER
     FELFINGKEI CNAYTELNNP IEQRMRFKMQ AQDINDGDDE AMITDEDFCV ALEYGLPPTG
     GFGMGIDRLT MFMTDAANIK DVILFPAMKP ESDN