ID   SYK_BRUME               Reviewed;         551 AA.
AC   Q8YCM8;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=BMEII0500;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; AE008918; AAL53742.1; -; Genomic_DNA.
DR   PIR; AC3572; AC3572.
DR   RefSeq; WP_004682127.1; NZ_GG703779.1.
DR   AlphaFoldDB; Q8YCM8; -.
DR   SMR; Q8YCM8; -.
DR   STRING; 224914.BMEII0500; -.
DR   EnsemblBacteria; AAL53742; AAL53742; BMEII0500.
DR   GeneID; 29595932; -.
DR   KEGG; bme:BMEII0500; -.
DR   PATRIC; fig|224914.52.peg.2872; -.
DR   eggNOG; COG1384; Bacteria.
DR   OMA; DWPMRWA; -.
DR   PhylomeDB; Q8YCM8; -.
DR   Proteomes; UP000000419; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..551
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152738"
FT   MOTIF           54..62
FT                   /note="'HIGH' region"
FT   MOTIF           303..307
FT                   /note="'KMSKS' region"
FT   BINDING         306
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00177"
SQ   SEQUENCE   551 AA;  61699 MW;  FC55DB8B469E1AC0 CRC64;
     MTSHRLPEIT LTPELTAAAA EAKAWPFEEA RKILKRYAKT GLPETVIFET GYGPSGLPHI
     GTFGEVARTS MVRHAFRILT QDKVKTRLIC FSDDLDGMRK VPDNVPDRAA LEPYLQMPLS
     SVPNPFGGDY KSFAEHNNAM LCRFLDTFGF DYEFASATEY YRSGRFDTVL LKAVERYDDI
     MKVMLPTLGE ERQATYSPFL PISPKSGRVL YVPMKKVDAK AGTITFDDED GEETTLSVTG
     GKVKLQWKPD FGMRWAALGV DFEMFGKDHQ TNAGIYDRIC EILGGRAPEH FVYELFLDQL
     GQKISKSKGN GISIDEWLAY APTESLTLYN FQKPKTAKKL YFDVIPKTVD EYFTYLSAYA
     RQEWKDRLNN PVWHIHYGNP PKADLPVTFA LMLNLVSASN AESPAVLWGF ISRHVPGVTP
     ENNPELDALV GYAIRYFNDF VKPTKKFRAP DDVERAALEA LDAKLGELPA GTDGNVIQNA
     ILDVARAIER YQDHTKKSPE GGPGVSVSFF QMLYEVLIGQ ERGPRFGSFV ALYGIDETRA
     LIKKALAGEL A