ID   SYK_CAUVN               Reviewed;         552 AA.
AC   B8GXH3;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=CCNA_00082;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; CP001340; ACL93549.1; -; Genomic_DNA.
DR   RefSeq; WP_010917973.1; NC_011916.1.
DR   RefSeq; YP_002515457.1; NC_011916.1.
DR   AlphaFoldDB; B8GXH3; -.
DR   SMR; B8GXH3; -.
DR   PRIDE; B8GXH3; -.
DR   EnsemblBacteria; ACL93549; ACL93549; CCNA_00082.
DR   GeneID; 7332173; -.
DR   KEGG; ccs:CCNA_00082; -.
DR   PATRIC; fig|565050.3.peg.82; -.
DR   HOGENOM; CLU_025562_2_0_5; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 256927at2; -.
DR   PhylomeDB; B8GXH3; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..552
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_1000199265"
FT   MOTIF           72..80
FT                   /note="'HIGH' region"
FT   MOTIF           320..324
FT                   /note="'KMSKS' region"
FT   BINDING         323
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00177"
SQ   SEQUENCE   552 AA;  61026 MW;  A64727130E6BD6A1 CRC64;
     MFEGLSPLAR DARSWPFEQA RATIARVLRV RLPDRADQDA AKALIDAGKT DEAVKAYPAL
     AKAVIFETGY GPSGLPHLGT FGEVARTTMV RQAFRALTDE AIPTRLIAFS DDMDGLRKVP
     DNIENKQPLI EDLGKPLTVV RDPFGTHDSF GAHNNARLRA FLDGFGFEYE FVSSTDCYKG
     GLFDATLLTA LERFDAIQKV MLPTLGEERR ATYSPFLPIS PSTGKVLQVP TLERNVEKGT
     IVFEDEDGSK VEVPVTGGHV KMQWKPDWAM RWTALGVDYE MSGKDLIDSV KASGAICKAL
     GGVPPEGFNY ELFLDENNQK ISKSKGNGLS MEDWLRYGAP ESLSYYMFQS PKSAKKLYFD
     VIPKASDEYL QQLDGFGRQE PAKQLDNPVW HIHGGKPPQQ GSPVSFSLML NLVSAADAST
     KEILWGFLSR YIPGASPETQ PLLDRLAGYA INYYEDFVKP SKVFRAPSDQ ERAAMLDLLA
     KLKAMPAGTQ DAELIQNEVF EVGKTHGFDP LRAWFQALYE VLLGQSQGPR FGSFAAIFGI
     DRTVALIEEK LG