BLH2_ARATH
ID BLH2_ARATH Reviewed; 739 AA.
AC Q9SW80; F4JQI6; O23196;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 3.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=BEL1-like homeodomain protein 2;
DE Short=BEL1-like protein 2;
DE AltName: Full=Protein SAWTOOTH 1;
GN Name=BLH2; Synonyms=SAW1; OrderedLocusNames=At4g36870;
GN ORFNames=AP22.90, C7A10.490;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Pidkowich M.S., Samach A., Modrusan Z., Haughn G.W.;
RT "A family of bel1-like homeodomain (BLH) proteins in Arabidopsis
RT thaliana.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=15120075; DOI=10.1016/j.cub.2004.04.032;
RA Smith H.M.S., Campbell B.C.C., Hake S.;
RT "Competence to respond to floral inductive signals requires the homeobox
RT genes PENNYWISE and POUND-FOOLISH.";
RL Curr. Biol. 14:812-817(2004).
RN [7]
RP INTERACTION WITH OFP1; OFP2; OFP4 AND OFP5.
RX PubMed=15781858; DOI=10.1073/pnas.0501181102;
RA Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.;
RT "A central role of Arabidopsis thaliana ovate family proteins in networking
RT and subcellular localization of 3-aa loop extension homeodomain proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005).
RN [8]
RP INTERACTION WITH TALE/KNOX PROTEINS, AND TISSUE SPECIFICITY.
RX PubMed=17873098; DOI=10.1105/tpc.106.048769;
RA Kumar R., Kushalappa K., Godt D., Pidkowich M.S., Pastorelli S.,
RA Hepworth S.R., Haughn G.W.;
RT "The Arabidopsis BEL1-LIKE HOMEODOMAIN proteins SAW1 and SAW2 act
RT redundantly to regulate KNOX expression spatially in leaf margins.";
RL Plant Cell 19:2719-2735(2007).
RN [9]
RP INTERACTION WITH KNATM.
RX PubMed=18398054; DOI=10.1105/tpc.108.058495;
RA Magnani E., Hake S.;
RT "KNOX lost the OX: the Arabidopsis KNATM gene defines a novel class of KNOX
RT transcriptional regulators missing the homeodomain.";
RL Plant Cell 20:875-887(2008).
CC -!- FUNCTION: Transcription factor that establishes leaf shape by
CC repressing growth in specific subdomains of the leaf. Negatively
CC regulates knox homeobox gene KNAT1/BP expression.
CC -!- SUBUNIT: May form heterodimeric complexes with TALE/KNOX proteins STM,
CC KNAT1/BP, KNAT2 and KNAT5 (PubMed:17873098). Interacts OFP1, OFP2, OFP4
CC and OFP5 (PubMed:15781858). Interacts with KNATM, isoform KNATM-B
CC (PubMed:18398054). {ECO:0000269|PubMed:15781858,
CC ECO:0000269|PubMed:17873098, ECO:0000269|PubMed:18398054}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in lateral organs.
CC {ECO:0000269|PubMed:17873098}.
CC -!- DOMAIN: The SR/KY and BELL domains are responsive for the interaction
CC between the TALE/BELL proteins and the TALE/KNOX proteins.
CC -!- SIMILARITY: Belongs to the TALE/BELL homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB16801.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80353.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF173816; AAD51349.1; -; mRNA.
DR EMBL; Z99707; CAB16801.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161590; CAB80353.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86711.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86712.1; -; Genomic_DNA.
DR EMBL; AY050459; AAK91472.1; -; mRNA.
DR PIR; D85435; D85435.
DR RefSeq; NP_001031797.4; NM_001036720.5.
DR RefSeq; NP_195405.4; NM_119851.6.
DR AlphaFoldDB; Q9SW80; -.
DR SMR; Q9SW80; -.
DR BioGRID; 15121; 16.
DR IntAct; Q9SW80; 12.
DR STRING; 3702.AT4G36870.2; -.
DR iPTMnet; Q9SW80; -.
DR PaxDb; Q9SW80; -.
DR PRIDE; Q9SW80; -.
DR ProteomicsDB; 240700; -.
DR EnsemblPlants; AT4G36870.1; AT4G36870.1; AT4G36870.
DR EnsemblPlants; AT4G36870.2; AT4G36870.2; AT4G36870.
DR GeneID; 829840; -.
DR Gramene; AT4G36870.1; AT4G36870.1; AT4G36870.
DR Gramene; AT4G36870.2; AT4G36870.2; AT4G36870.
DR KEGG; ath:AT4G36870; -.
DR Araport; AT4G36870; -.
DR TAIR; locus:2115000; AT4G36870.
DR eggNOG; KOG0773; Eukaryota.
DR HOGENOM; CLU_011058_2_0_1; -.
DR InParanoid; Q9SW80; -.
DR OrthoDB; 265092at2759; -.
DR PRO; PR:Q9SW80; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SW80; baseline and differential.
DR Genevisible; Q9SW80; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009965; P:leaf morphogenesis; IGI:TAIR.
DR GO; GO:0048363; P:mucilage pectin metabolic process; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR006563; POX_dom.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF07526; POX; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00574; POX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..739
FT /note="BEL1-like homeodomain protein 2"
FT /id="PRO_0000315458"
FT DNA_BIND 498..560
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 23..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..332
FT /note="SR/KY domain"
FT /evidence="ECO:0000250"
FT REGION 341..378
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 376..447
FT /note="BELL domain"
FT /evidence="ECO:0000250"
FT REGION 567..627
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 596..621
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 485
FT /note="N -> Q (in Ref. 1; AAD51349 and 5; AAK91472)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 739 AA; 81605 MW; FF4323A165ABC4E2 CRC64;
MGITKTSPNT TILLKTFHNN SMSQDYHHHH HHNQHQGGIF NFSNGFDRSD SPNLTTQQKQ
EHQRVEMDEE SSVAGGRIPV YESAGMLSEM FNFPGSSGGG RDLDLGQSFR SNRQLLEEQH
QNIPAMNATD SATATAAAMQ LFLMNPPPPQ QPPSPSSTTS PRSHHNSSTL HMLLPSPSTN
TTHHQNYTNH MSMHQLPHQH HQQISTWQSS PDHHHHHHNS QTEIGTVHVE NSGGHGGQGL
SLSLSSSLEA AAKAEEYRNI YYGANSSNAS PHHQYNQFKT LLANSSQHHH QVLNQFRSSP
AASSSSMAAV NILRNSRYTT AAQELLEEFC SVGRGFLKKN KLGNSSNPNT CGGDGGGSSP
SSAGANKEHP PLSASDRIEH QRRKVKLLTM LEEVDRRYNH YCEQMQMVVN SFDIVMGHGA
ALPYTALAQK AMSRHFRCLK DAVAAQLKQS CELLGDKDAA GISSSGLTKG ETPRLRLLEQ
SLRQNRAFHQ MGMMEQEAWR PQRGLPERSV NILRAWLFEH FLHPYPSDAD KHLLARQTGL
SRNQVSNWFI NARVRLWKPM VEEMYQQESK EREREEELEE NEEDQETKNS NDDKSTKSNN
NESNFTAVRT TSQTPTTTAP DASDADAAVA TGHRLRSNIN AYENDASSLL LPSSYSNAAA
PAAVSDDLNS RYGGSDAFSA VATCQQSVGG FDDADMDGVN VIRFGTNPTG DVSLTLGLRH
AGNMPDKDAS FCVREFGGF
