ID   SYK_HALMA               Reviewed;         545 AA.
AC   Q5UXX4;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=rrnAC3173;
OS   Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM
OS   B-1809) (Halobacterium marismortui).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=272569;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809;
RX   PubMed=15520287; DOI=10.1101/gr.2700304;
RA   Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W.,
RA   Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E.,
RA   Hood L., Ng W.V.;
RT   "Genome sequence of Haloarcula marismortui: a halophilic archaeon from the
RT   Dead Sea.";
RL   Genome Res. 14:2221-2234(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; AY596297; AAV47879.1; -; Genomic_DNA.
DR   RefSeq; WP_011224652.1; NZ_CP039138.1.
DR   AlphaFoldDB; Q5UXX4; -.
DR   SMR; Q5UXX4; -.
DR   STRING; 272569.rrnAC3173; -.
DR   PRIDE; Q5UXX4; -.
DR   EnsemblBacteria; AAV47879; AAV47879; rrnAC3173.
DR   GeneID; 40153980; -.
DR   KEGG; hma:rrnAC3173; -.
DR   PATRIC; fig|272569.17.peg.3713; -.
DR   eggNOG; arCOG00485; Archaea.
DR   HOGENOM; CLU_025562_1_0_2; -.
DR   OMA; DWPMRWA; -.
DR   Proteomes; UP000001169; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 6.10.20.10; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..545
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152749"
FT   MOTIF           42..50
FT                   /note="'HIGH' region"
FT   MOTIF           307..311
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   545 AA;  61356 MW;  8E93E59C1E36DD35 CRC64;
     MAEDPYEVGR GVSRAFWADS VADAIEARDP DEPIVIKGGV SPSGVPHIGH FNEIMRGYYV
     AEALRDRGHE VRQVFTADDK DRLRAVPRQL ADLDWNVVGL GEVDAGALGR NLGKPYTDIP
     DPFGCCDSYG AHFTALLQKS AELVGVDVEF VSNTELYADG EFEAVTRRVL ERADRARDVL
     AEYQNKVDDD YVPFLPQCAE CGKITEGVTA VDLDAGEVEY VCEDVEAGDQ TIDGCGHEGT
     ATLRDGKLPW RFEWPAQWEI LGVDFEPFGK DHAEGSWPSG EDVAENVLDI QPPVPMVYEW
     FTLDGEPLSS SSGNVITVDE VLDILEPEVF KYFFVKDPRK QRDFSVENID QLVDEFDRFE
     RRYFEEIEAS EDDAELAERA YPMVVDDPRE QRIRIPYTFA AVLGMTDDPA LREQIARKEG
     HIPDDAPEWA VEQALARVER AQEWASLTNN EFNYELKRAE IPEVSFDDAT AAALDELADF
     IAEGHDGEAI QSEIYETAKR NDIDISEFFS AGYRLLFDDT EGPQLGTFIA KLDREFVVER
     FRRNE