SYK_HALSA
ID SYK_HALSA Reviewed; 548 AA.
AC Q9HNN7;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=VNG_2017G;
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=64091;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR EMBL; AE004437; AAG20183.1; -; Genomic_DNA.
DR PIR; C84352; C84352.
DR RefSeq; WP_010903484.1; NC_002607.1.
DR AlphaFoldDB; Q9HNN7; -.
DR SMR; Q9HNN7; -.
DR STRING; 64091.VNG_2017G; -.
DR PaxDb; Q9HNN7; -.
DR PRIDE; Q9HNN7; -.
DR EnsemblBacteria; AAG20183; AAG20183; VNG_2017G.
DR GeneID; 5954176; -.
DR KEGG; hal:VNG_2017G; -.
DR PATRIC; fig|64091.14.peg.1540; -.
DR HOGENOM; CLU_025562_1_0_2; -.
DR InParanoid; Q9HNN7; -.
DR OMA; DWPMRWA; -.
DR OrthoDB; 9880at2157; -.
DR PhylomeDB; Q9HNN7; -.
DR Proteomes; UP000000554; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.350; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR InterPro; IPR002904; Lys-tRNA-ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR37940; PTHR37940; 1.
DR Pfam; PF01921; tRNA-synt_1f; 1.
DR SUPFAM; SSF48163; SSF48163; 1.
DR TIGRFAMs; TIGR00467; lysS_arch; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..548
FT /note="Lysine--tRNA ligase"
FT /id="PRO_0000152750"
FT MOTIF 43..51
FT /note="'HIGH' region"
FT MOTIF 308..312
FT /note="'KMSKS' region"
SQ SEQUENCE 548 AA; 60875 MW; 85DC68AC77F7FD48 CRC64;
MHPDDDPYAD DGSGRLFWAD AAADRVLDGD PEEPIVIKGG ISPSGVPHLG NMNEIMRGYF
VAAVLRERGH EVRQVFTSDD RDPLRGLPRK LADLDGEVVA LGDVNAGALG QNLGAPYTEI
PDPFGCCDSY GAHFSNLIQS SADRLGVDVE MVSNTETYAN GGFEEVTRYL LEHVTAARDV
LGEYQDKVDE EYVPFNPICE ACGKVTETVT DVDADAGTVE YVCTDMDAGD QTIEGCGHEG
TATLREGKLP WRFEWPAQWR TLGVDYEPFG KDHAEGSWPS GTDISRNVLG DEPPTPMVYE
WFTLDGEPFS SSAGHVILVQ DVLALLELPV VRYFFSKDPR RARDFSIEHL DQLVDEFDRM
ERVYFGEAAD ASEDERERAA RVYPLLVDDI DADRVRIPFT FAAVLGMTED SALREEIARR
EGHIPADADA DTVADALTRV ERAREWARRT NNEFNYRLAR AEIPAHDFDD ATAAALDELA
AFLDDEDPDG EALQGEIYEA AKRHDIDIGA FFAAGYRLFF DEDQGPKLGP FLAKLDREFV
VTRLRREG
