BLH_AGRFC
ID BLH_AGRFC Reviewed; 431 AA.
AC Q8UAA9; Q7CSJ2;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Beta-lactamase hydrolase-like protein {ECO:0000303|PubMed:17586627};
DE Short=BLH {ECO:0000303|PubMed:17586627};
DE EC=3.-.-.-;
GN Name=blh {ECO:0000303|PubMed:17586627}; OrderedLocusNames=Atu3465;
GN ORFNames=AGR_L_2726;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3]
RP INDUCTION BY BIGR.
RC STRAIN=C58 / ATCC 33970;
RX PubMed=17586627; DOI=10.1128/jb.00331-07;
RA Barbosa R.L., Benedetti C.E.;
RT "BigR, a transcriptional repressor from plant-associated bacteria,
RT regulates an operon implicated in biofilm growth.";
RL J. Bacteriol. 189:6185-6194(2007).
CC -!- FUNCTION: Could play a role in cell adherence or biofilm development.
CC {ECO:0000305|PubMed:17586627}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P25910};
CC -!- INDUCTION: Repressed by BigR. {ECO:0000269|PubMed:17586627}.
CC -!- MISCELLANEOUS: Part of an operon that comprises at least itself and
CC bigR. {ECO:0000269|PubMed:17586627}.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC {ECO:0000305}.
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DR EMBL; AE007870; AAK89929.1; -; Genomic_DNA.
DR PIR; AH2982; AH2982.
DR PIR; G98300; G98300.
DR RefSeq; NP_357144.1; NC_003063.2.
DR RefSeq; WP_010973062.1; NC_003063.2.
DR AlphaFoldDB; Q8UAA9; -.
DR SMR; Q8UAA9; -.
DR STRING; 176299.Atu3465; -.
DR EnsemblBacteria; AAK89929; AAK89929; Atu3465.
DR KEGG; atu:Atu3465; -.
DR PATRIC; fig|176299.10.peg.3304; -.
DR eggNOG; COG0491; Bacteria.
DR eggNOG; COG3453; Bacteria.
DR HOGENOM; CLU_030571_9_0_5; -.
DR OMA; LYLCHDY; -.
DR PhylomeDB; Q8UAA9; -.
DR BioCyc; AGRO:ATU3465-MON; -.
DR Proteomes; UP000000813; Chromosome linear.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050313; F:sulfur dioxygenase activity; IEA:InterPro.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR CDD; cd07724; POD-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; -; 1.
DR Gene3D; 3.90.190.10; -; 1.
DR InterPro; IPR005939; BLH_phosphatase-like.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR044528; POD-like_MBL-fold.
DR InterPro; IPR029021; Prot-tyrosine_phosphatase-like.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR Pfam; PF04273; DUF442; 1.
DR Pfam; PF00753; Lactamase_B; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF52799; SSF52799; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
DR TIGRFAMs; TIGR01244; TIGR01244; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Metal-binding; Reference proteome; Zinc.
FT CHAIN 1..431
FT /note="Beta-lactamase hydrolase-like protein"
FT /id="PRO_0000305338"
FT BINDING 212
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 286
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 309
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P25910"
SQ SEQUENCE 431 AA; 47163 MW; 9A580126DA97374B CRC64;
MKAVRINERL TIAGQPMIAD FPSLSAQGFK SIINARPDGE EPGQPGNTQE KSAAGAAGMD
YGFIPVSGPT ITEADIRAFQ QKMAEAEGPV FAHCKGGTRA LTLYVLGEAL DGRIQRSDIE
DFGKTHGFDL CAATRWLERQ SAAVPHIKAF FDPRTWSVQY VVSDPATGGC AIIDPVYDFD
EKSGATGTMN ADAILDYVKR HGLSVEWILD THPHADHFSA ADYLKQKTGA KTAIGAKVTG
VQKLWQEKYN WSDFKTDGSQ WDQLFEAGDR FSIGSLEARV LFSPGHTLAS VTYVVGNAAF
VHDTLFMPDS GTARADFPGG SAKQLWASIQ DILALPDDTR LFTGHDYQPG GRAPKWESTV
GEQTRSNPHL AGMTEEDFVR LREARDRTLP MPKLILHALQ VNIRGGRLPE PEANGKHYLK
FPLDVLEGST W
