ID   SYK_METBF               Reviewed;         537 AA.
AC   Q46CD2;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=Mbar_A1507;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; CP000099; AAZ70460.1; -; Genomic_DNA.
DR   RefSeq; WP_011306506.1; NC_007355.1.
DR   AlphaFoldDB; Q46CD2; -.
DR   SMR; Q46CD2; -.
DR   STRING; 269797.Mbar_A1507; -.
DR   EnsemblBacteria; AAZ70460; AAZ70460; Mbar_A1507.
DR   GeneID; 3627694; -.
DR   KEGG; mba:Mbar_A1507; -.
DR   eggNOG; arCOG00485; Archaea.
DR   HOGENOM; CLU_025562_1_0_2; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 9880at2157; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 6.10.20.10; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..537
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000225642"
FT   MOTIF           30..38
FT                   /note="'HIGH' region"
FT   MOTIF           276..280
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   537 AA;  60158 MW;  29D2F4F72F657F58 CRC64;
     MADTIHWADV IAEDVLKKSG KHLVATGITP SGNIHIGNMR EVVTADAVYR ALSNKGANAD
     FIYIADNYDP LRKVYPFLPE SYVEHVGKPI SEIPCPCGNC ANYAEHFLKP FLEALRRLGI
     NPQVYRADEM YKTGKYTEAI KTALVKRDAI AKILEEVSGK TVAADWSPFN PRCNQCGKIT
     TTKVTGFDLE AETVDYVCAC GHSGIVPMAG GGKLTWRVDW PARWSVLGVT VEPFGKDHAS
     KGGSYDTGKR IVREIFGHEP PYPIVYEWIM LGKQGAMSSS TGVVISISDM LEIIPPEVLR
     YLIIRTKPEK HIQFDPGQPL LTLVDEYERL RTQFRENDPS LGTFQKRVYE LSRATGICQS
     EIPFKQMVTI YQVARGDFAQ ILKIVKRSGF STEDKKCIKE LADNVSKWLK LYAPPFAKFK
     VKEKVPVQAA TLSELQRAFL SAFAALIESR GEISGEEYHM LVYSAKDEGS ELNRKIAEKL
     NTHAPQVDPR ELFKAIYFSL LGQKSGPKAG WFLSSFDKEF LVERFEEASN YSPEKQA