ID   SYK_METKA               Reviewed;         555 AA.
AC   Q8TWP6;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=MK0986;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; AE009439; AAM02199.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TWP6; -.
DR   SMR; Q8TWP6; -.
DR   STRING; 190192.MK0986; -.
DR   EnsemblBacteria; AAM02199; AAM02199; MK0986.
DR   KEGG; mka:MK0986; -.
DR   PATRIC; fig|190192.8.peg.1033; -.
DR   HOGENOM; CLU_025562_1_0_2; -.
DR   OMA; DWPMRWA; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 6.10.20.10; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..555
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152754"
FT   MOTIF           37..45
FT                   /note="'HIGH' region"
FT   MOTIF           301..305
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   555 AA;  63462 MW;  A06B1DF2DB4B8E80 CRC64;
     MTKEHWSEVK AREVTEHCRK HADELPDEIV VASGASTSGR LHVGNARDVL TADAVARVLR
     ERYHEDVRVV WISDDVDPLR RIPRDLDGRL SEDYLGVPYK AIPVGDEPYS DRWARNFVEE
     LREFGAEVEW ISSAELYTDN GFVKLVREVV NDYYGGGGRL ASVLERFGLE DARVYMPVCE
     ECGRIATTRV VDVDGWRIEY VCEGRHEIGD AVLEGCGHRG ELDLRKPIEV NGFEIPPGKL
     GWKIEWPTRW VYLGVACEPF GKDHYVAGGS YEVGSAIAEE FFGFPAPVPV PYEWITLDGK
     AMSSSKGHYV TLSDWGEVCH REVLRYLVLR GKPLKHLDLD LRFGLLQAVD DYDELEKRYF
     AGEADERERR IYELSRVDEI PEECPPHVPF RFCAVVAQVV GIEDDVSEEE FERALEIFRR
     TGHLEAEPEG FGREWLRERL EKASRWVDRY APEEARFRVR EEPEPVELSD KEREFLEDLV
     RRLESETTKE DPETLQRTVF EAARTAGLRP ADAFRVFYNV VVGKDRGPRA GTLIAAVGVD
     RISRLIRGCL EASED