BLH_XYLFA
ID BLH_XYLFA Reviewed; 431 AA.
AC Q9PFB0;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Beta-lactamase hydrolase-like protein {ECO:0000303|PubMed:17586627};
DE Short=BLH {ECO:0000303|PubMed:17586627};
DE EC=3.-.-.-;
GN Name=blh {ECO:0000303|PubMed:17586627}; OrderedLocusNames=XF_0768;
OS Xylella fastidiosa (strain 9a5c).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xylella.
OX NCBI_TaxID=160492;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9a5c;
RX PubMed=10910347; DOI=10.1038/35018003;
RA Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M.,
RA Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S.,
RA Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S.,
RA Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H.,
RA Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M.,
RA Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H.,
RA Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S.,
RA Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H.,
RA Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D.,
RA Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E.,
RA Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F.,
RA Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N.,
RA Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F.,
RA Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y.,
RA Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O.,
RA Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A.,
RA de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R.,
RA Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B.,
RA Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G.,
RA Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M.,
RA da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z.,
RA Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D.,
RA Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S.,
RA Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.;
RT "The genome sequence of the plant pathogen Xylella fastidiosa.";
RL Nature 406:151-159(2000).
RN [2]
RP INDUCTION BY BIGR.
RC STRAIN=9a5c;
RX PubMed=17586627; DOI=10.1128/jb.00331-07;
RA Barbosa R.L., Benedetti C.E.;
RT "BigR, a transcriptional repressor from plant-associated bacteria,
RT regulates an operon implicated in biofilm growth.";
RL J. Bacteriol. 189:6185-6194(2007).
CC -!- FUNCTION: Could play a role in cell adherence or biofilm development.
CC {ECO:0000305|PubMed:17586627}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P25910};
CC -!- INDUCTION: Repressed by BigR. {ECO:0000269|PubMed:17586627}.
CC -!- MISCELLANEOUS: Part of an operon that comprises bigR, XF_0764, XF_0765
CC and XF_0766. {ECO:0000269|PubMed:17586627}.
CC -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC {ECO:0000305}.
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DR EMBL; AE003849; AAF83578.1; -; Genomic_DNA.
DR PIR; E82766; E82766.
DR RefSeq; WP_010893291.1; NC_002488.3.
DR AlphaFoldDB; Q9PFB0; -.
DR SMR; Q9PFB0; -.
DR STRING; 160492.XF_0768; -.
DR EnsemblBacteria; AAF83578; AAF83578; XF_0768.
DR KEGG; xfa:XF_0768; -.
DR eggNOG; COG0491; Bacteria.
DR eggNOG; COG3453; Bacteria.
DR HOGENOM; CLU_030571_9_1_6; -.
DR OMA; LYLCHDY; -.
DR Proteomes; UP000000812; Chromosome.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050313; F:sulfur dioxygenase activity; IEA:InterPro.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR CDD; cd07724; POD-like_MBL-fold; 1.
DR Gene3D; 3.60.15.10; -; 1.
DR Gene3D; 3.90.190.10; -; 1.
DR InterPro; IPR005939; BLH_phosphatase-like.
DR InterPro; IPR001279; Metallo-B-lactamas.
DR InterPro; IPR044528; POD-like_MBL-fold.
DR InterPro; IPR029021; Prot-tyrosine_phosphatase-like.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR Pfam; PF04273; DUF442; 1.
DR Pfam; PF00753; Lactamase_B; 1.
DR SMART; SM00849; Lactamase_B; 1.
DR SUPFAM; SSF52799; SSF52799; 1.
DR SUPFAM; SSF56281; SSF56281; 1.
DR TIGRFAMs; TIGR01244; TIGR01244; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Metal-binding; Reference proteome; Zinc.
FT CHAIN 1..431
FT /note="Beta-lactamase hydrolase-like protein"
FT /id="PRO_0000305339"
FT BINDING 212
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 286
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P25910"
FT BINDING 309
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P25910"
SQ SEQUENCE 431 AA; 47530 MW; C027E5A72E80FD47 CRC64;
MRIVDINERL AISGQPNTDE FINFARRGYR SIINLRPDGE EPNQPGNDAE QAAARRAGLA
YNFVPVIGTS ITEADIQAFQ RAIATTEGSV LVHCKSGTRA LMLYALSEVI DGRMKRDEVE
ALGHAHGFDL GRAVTWLERQ AIQTPRVSGF FDPRTSSIQY VVTDQTTKRC AIIDPVLDFD
EKSGATATTN ADAILAHVEQ QGLTVEWILD THPHADHFSA AQYLKQRTGA PTAIGTHVTE
VQRLWREIYN WPTLSANGSQ WDHLFADGDV FNVGSIKGRV MFSPGHTLAS VTYVIGDTAF
VHDTIFMPDA GTARADFPGG SARALWSSIQ TILSLPDETR LFTGHDYQPS GRHPRWESTV
GEQKKANPHL AGVDETTFVA LREARDKTLP MPKLILHALQ VNVLGGRLPE PETNGRRYLK
FPLNALEGAA W
