ID   SYK_METTH               Reviewed;         544 AA.
AC   O27585;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Lysine--tRNA ligase;
DE            EC=6.1.1.6;
DE   AltName: Full=Lysyl-tRNA synthetase;
DE            Short=LysRS;
GN   Name=lysS; OrderedLocusNames=MTH_1542;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC   -!- INTERACTION:
CC       O27585; O27552: leuS; NbExp=3; IntAct=EBI-7963255, EBI-7963108;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000666; AAB86016.1; -; Genomic_DNA.
DR   PIR; G69072; G69072.
DR   AlphaFoldDB; O27585; -.
DR   SMR; O27585; -.
DR   IntAct; O27585; 1.
DR   MINT; O27585; -.
DR   STRING; 187420.MTH_1542; -.
DR   EnsemblBacteria; AAB86016; AAB86016; MTH_1542.
DR   KEGG; mth:MTH_1542; -.
DR   PATRIC; fig|187420.15.peg.1505; -.
DR   HOGENOM; CLU_025562_1_0_2; -.
DR   OMA; DWPMRWA; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR045462; aa-tRNA-synth_I_cd-bd.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF19269; Anticodon_2; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
PE   1: Evidence at protein level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..544
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152757"
FT   MOTIF           48..56
FT                   /note="'HIGH' region"
FT   MOTIF           292..296
FT                   /note="'KMSKS' region"
FT   BINDING         295
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   544 AA;  63666 MW;  D033EABDDEAA21CF CRC64;
     MKFTHFFVAL ILSRFLQVIS LKHWIERIAD ELKERDVEEH VVASGTSISG SIHIGNSCDV
     FIASSIAKSL KKDGFKSRTV WIADDHDPLR KVPYPLPESY EKYLGVPYSM IPCPEGCCES
     FVEHFQRPFL EALERFRIGV EHYSGARMYT EGLYNDYIRT SLERAPEIRE IFNRFRDRPL
     RDDWLPYNPI CEKCGRVNTT EAYDFSGDTV RYRCECGFDG EMDIKSGLGK LTWRVEWAAR
     WKILGVTCEP FGKDHAASGG SYDVSSIISE EIFDYPAPYP VPYEWITLRG EAMSKSRGVF
     FTPGQWLEIG PPESLNYFIF RSKPMKHKDF NPDMPFLDLM DQFDRTERIY YGMEDAASEK
     EEQKLRNIYR VSMIEEFDLP LRPSYRFMTV ACQIAGDDPE RLYDILRRNS QLPEELMDLE
     LDQLTDKQLE QLNERIENVK NWLRLYAPEF VKFQVQEELP DVELSEPQLK FLQDVADLME
     SREMSAEELH DEMYSILRRH GLKPQKAFQA IYRVLIGKKM GPRAASFLLS LERDFVIRRL
     RLEA