ID   SYK_PICTO               Reviewed;         504 AA.
AC   Q6L1V1;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=PTO0466;
OS   Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS   100828).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Picrophilaceae; Picrophilus.
OX   NCBI_TaxID=263820;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
RX   PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA   Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA   Schepers B., Dock C., Antranikian G., Liebl W.;
RT   "Genome sequence of Picrophilus torridus and its implications for life
RT   around pH 0.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; AE017261; AAT43051.1; -; Genomic_DNA.
DR   RefSeq; WP_011177267.1; NC_005877.1.
DR   AlphaFoldDB; Q6L1V1; -.
DR   SMR; Q6L1V1; -.
DR   STRING; 263820.PTO0466; -.
DR   EnsemblBacteria; AAT43051; AAT43051; PTO0466.
DR   GeneID; 2844802; -.
DR   KEGG; pto:PTO0466; -.
DR   PATRIC; fig|263820.9.peg.492; -.
DR   eggNOG; arCOG00485; Archaea.
DR   HOGENOM; CLU_025562_0_0_2; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 9880at2157; -.
DR   Proteomes; UP000000438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR045462; aa-tRNA-synth_I_cd-bd.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF19269; Anticodon_2; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..504
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000259352"
FT   MOTIF           23..31
FT                   /note="'HIGH' region"
SQ   SEQUENCE   504 AA;  58004 MW;  AA3528A01868E8D5 CRC64;
     MHWSESLLKD VSGDQRISTG ISPSGPIHIG NMREILTGDI IYKEALKLGI KASFIYLCDD
     MDPLRKVYPF LPGSYERYVG HPLSMIPAPD GDKTYSEYFL EPFKETIDKI DVRPEIISTT
     SLYKNGVLSR AIDIAMNNRE KIKNILNSIG NYKITGDWYP YEPVCKSCGR INTTTVISYN
     YPYAEYKCKC GYTGKADIRT DDGKMPWRVE WPAKWFSLHV TIEPFGKDHG AAGGSYDTGK
     AIASDIFNIN PPLPLLYERI FLKGKGVMHS STGVVIPASE MIKFSPPEII RFLIAKNNPG
     RHIDFDPGPG LLNLIDEYEK YERAYFGLDS VKDDDYKEVY ELSRLKILKE PEKITFRHIV
     TLVQIYNNND ALLSALKRSG YEKDYIDDYI MNEIDTARYW LEKYAPPEMK FSLTDENINL
     NDDERKIVNE FLENIDNIEW SPDSIHNYVY DIIGRSKMRP QDAFAVFYKI LIGRSRGPRL
     GYFIYNLGRE YIIKRFSSIL AETF