ID   SYK_RICCK               Reviewed;         522 AA.
AC   A8EZ69;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=A1E_03595;
OS   Rickettsia canadensis (strain McKiel).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; belli group.
OX   NCBI_TaxID=293613;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=McKiel;
RA   Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Dasch G., Eremeeva M.;
RT   "Complete genome sequence of Rickettsia canadensis.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; CP000409; ABV73652.1; -; Genomic_DNA.
DR   RefSeq; WP_012148847.1; NC_009879.1.
DR   AlphaFoldDB; A8EZ69; -.
DR   SMR; A8EZ69; -.
DR   STRING; 293613.A1E_03595; -.
DR   EnsemblBacteria; ABV73652; ABV73652; A1E_03595.
DR   KEGG; rcm:A1E_03595; -.
DR   eggNOG; COG1384; Bacteria.
DR   HOGENOM; CLU_025562_2_0_5; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 256927at2; -.
DR   Proteomes; UP000007056; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..522
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_1000040352"
FT   MOTIF           44..52
FT                   /note="'HIGH' region"
FT   MOTIF           290..294
FT                   /note="'KMSKS' region"
FT   BINDING         293
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00177"
SQ   SEQUENCE   522 AA;  60362 MW;  7EF6830ED69489E8 CRC64;
     MLEIWEDAIQ SNAWPFVEAK KILDSLNGKT PEKGYILFET GYGPSGLPHI GTFGENARMV
     MVQKAFEQLS SIPTKLICFS DDMDGLRKVP SNIPNSEMLA QYMDMPLTSI LDPFGEYESY
     GAYMNAKLRS FLDKFGFEYE FYSSTKCYKA GMFDEMLLKV LEKYDEIMEL MLPTFREERK
     ATYSPFMPIC PKTGKVLQVP IEKWDAKRGT VTYKDEDNNY IEVPVTGGHC KLQWKPDFGM
     RWAALKVDYE MYGKDHLANA RLYSEICRIL GGKPPVQLCY ELFLDENGEK ISKSKGNSIS
     VDDWLKYAPV ESMALFMYQN PTRAKRLFFD VIPQNVDAYI TFNQKYHLEE DRAKRFANPV
     YHIHHGHVPK IETFGITYSL LLNLTSVCNP SDKSVLWGFI SKYAPKATPN TTTYLDRLAE
     FAIRYYDDFI KAHKSYYTPS EKHKVILRDI LDMLSSISEE TEGEVIQKSI YDIGMKAGYE
     NLRDYFKDLY QILLGQNEGP RLGTFIKLYG IKEMKKLIER KL