ID   SYK_THEVO               Reviewed;         503 AA.
AC   Q979B8;
DT   25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Lysine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS {ECO:0000255|HAMAP-Rule:MF_00177}; OrderedLocusNames=TV1243;
GN   ORFNames=TVG1282914;
OS   Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC
OS   15438 / GSS1).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1;
RX   PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA   Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y.,
RA   Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T.,
RA   Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT   "Archaeal adaptation to higher temperatures revealed by genomic sequence of
RT   Thermoplasma volcanium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
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DR   EMBL; BA000011; BAB60385.1; -; Genomic_DNA.
DR   RefSeq; WP_010917477.1; NC_002689.2.
DR   AlphaFoldDB; Q979B8; -.
DR   SMR; Q979B8; -.
DR   STRING; 273116.14325481; -.
DR   EnsemblBacteria; BAB60385; BAB60385; BAB60385.
DR   GeneID; 1441359; -.
DR   KEGG; tvo:TVG1282914; -.
DR   eggNOG; arCOG00485; Archaea.
DR   HOGENOM; CLU_025562_0_0_2; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 9880at2157; -.
DR   PhylomeDB; Q979B8; -.
DR   Proteomes; UP000001017; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 6.10.20.10; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR045462; aa-tRNA-synth_I_cd-bd.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF19269; Anticodon_2; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..503
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152763"
FT   MOTIF           23..31
FT                   /note="'HIGH' region"
FT   MOTIF           267..271
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   503 AA;  56997 MW;  EF15FED06BC11B56 CRC64;
     MFWADAAVKD LIGEQRISTG ISPSGPIHVG NMREILTGDI LYKAVIKRGL KSDFIYLCDD
     MDPLRKVYPF LDQSYSKYVG FPLKNIPSPD GVGVYSDHFL NPFIEVMRKT GIPARVIKTS
     DLYGNGILAD AIDTVMERRS EIKDILEKIT GRMIEGDFYP YEPLCEKCGR INSTSVISYK
     YPYAEYTCKC GHHGFADIRR AEGKMPWRIE WPAKWYALKV SIEPFGKDHG APGGSYDTGK
     RIAREIFGIE PPLPLVYERI MLKGKGAMHS STGLAIPASE IIDVMPPELL RFLIARVNPS
     RHIDFDPGLG LLNLFDEFER YLNKYREGDI DGDSKAIIEY SLIEKEKLSY PIDFRHLITL
     IQIYQKKEDI LRAAMASTKD QLDESALYDE IKYAKNWLER YAPDNVKFKL LAIDEKAELS
     DQDRAILSKF LEVSNSMAWD SNEIHSTVHN VAKDLGIGPD AAFSTFYKVF IGKERGPRLG
     YFLFNLGKDF TIARIKSVLG GNS