ID   SYK_TREPA               Reviewed;         528 AA.
AC   O83650;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 130.
DE   RecName: Full=Lysine--tRNA ligase;
DE            EC=6.1.1.6;
DE   AltName: Full=Lysyl-tRNA synthetase;
DE            Short=LysRS;
GN   Name=lysS; OrderedLocusNames=TP_0644;
OS   Treponema pallidum (strain Nichols).
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=243276;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA   McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA   Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA   Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL   Science 281:375-388(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000520; AAC65618.1; -; Genomic_DNA.
DR   PIR; C71299; C71299.
DR   RefSeq; WP_010882089.1; NC_021490.2.
DR   AlphaFoldDB; O83650; -.
DR   SMR; O83650; -.
DR   IntAct; O83650; 3.
DR   STRING; 243276.TPANIC_0644; -.
DR   EnsemblBacteria; AAC65618; AAC65618; TP_0644.
DR   GeneID; 57879169; -.
DR   KEGG; tpa:TP_0644; -.
DR   eggNOG; COG1384; Bacteria.
DR   HOGENOM; CLU_025562_1_0_12; -.
DR   OMA; DWPMRWA; -.
DR   OrthoDB; 256927at2; -.
DR   BRENDA; 6.1.1.6; 6429.
DR   Proteomes; UP000000811; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 6.10.20.10; -; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR37940; PTHR37940; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; SSF48163; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..528
FT                   /note="Lysine--tRNA ligase"
FT                   /id="PRO_0000152745"
FT   MOTIF           36..44
FT                   /note="'HIGH' region"
FT   MOTIF           287..291
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   528 AA;  60901 MW;  B90425263C4EBF6D CRC64;
     MSICEKSLHW ADKVAHKIIK ERADCDQYTC ASGITPSGTV HIGNFREIIS VDLVVRALRD
     QGKSVRFVHS WDDYDVFRRI PDNVPAQDEL KQYIRMPITS VPDPFQQEDS YARHHEREIE
     SALPEVGIYP EYVYQSKQYQ AGVYAQEIKI ALDNRHRIQA ILNEYRDEQH KISGTYWPVS
     VFCTACHKDC TTVDAWDSHW CLQYHCECGH GEQVDLRQTS AVKLSWRVDW AMRWSKEHVV
     FEPAGKDHHS QGGSFDTARL ISDHIYHWPA PVSFRYDFIG LKGLPGKMSS SAGKVVGLRD
     VLEVYQPEVL RYLFVSTRPN TEFSISFDLD VLKIYEDYDK SERVAWGIHA AKSEHEFMRH
     KRIYELSQVR GMPPCISYQV PFRHVCNILQ INSGDISAVL AFFSDIHKDQ IERFVRRCQC
     AWNWIRDAGA PDDFKFTLKE DGVRVPLSAE ITEALRLIRD TLVPRTDVLS EKELSAELYA
     VARQIPVGSK ELFTALYQVL IGKNQGPRLA GFMKVIGTQR LHRMLSVY