ID   BLIP_STRCL              Reviewed;         201 AA.
AC   P35804;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Beta-lactamase inhibitory protein;
DE            Short=BLIP;
DE   Flags: Precursor;
OS   Streptomyces clavuligerus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2203736; DOI=10.1128/jb.172.9.4909-4918.1990;
RA   Doran J.L., Leskiw B.K., Aippersbach S., Jensen S.E.;
RT   "Isolation and characterization of a beta-lactamase-inhibitory protein from
RT   Streptomyces clavuligerus and cloning and analysis of the corresponding
RT   gene.";
RL   J. Bacteriol. 172:4909-4918(1990).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
RX   PubMed=8145854; DOI=10.1038/368657a0;
RA   Strynadka N.C.J., Jensen S.E., Johns K., Blanchard H., Page M., Matagne A.,
RA   Frere J.-M., James M.N.G.;
RT   "Structural and kinetic characterization of a beta-lactamase-inhibitor
RT   protein.";
RL   Nature 368:657-660(1994).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF TEM-1 COMPLEXED WITH BLIP.
RX   PubMed=8605632; DOI=10.1038/nsb0396-290;
RA   Strynadka N.C.J., Jensen S.E., Alzari P.M., James M.N.G.;
RT   "A potent new mode of beta-lactamase inhibition revealed by the 1.7 A X-ray
RT   crystallographic structure of the TEM-1-BLIP complex.";
RL   Nat. Struct. Biol. 3:290-297(1996).
CC   -!- FUNCTION: Inhibits a wide variety of beta lactamases.
CC   -!- INTERACTION:
CC       P35804; P62593: blaT-6; Xeno; NbExp=2; IntAct=EBI-1031985, EBI-1031989;
CC   -!- SUBCELLULAR LOCATION: Secreted.
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DR   EMBL; M34538; AAA16182.1; -; Genomic_DNA.
DR   PIR; A36710; A36710.
DR   RefSeq; WP_044955856.1; NZ_CP032052.1.
DR   PDB; 1JTG; X-ray; 1.73 A; B/D=37-201.
DR   PDB; 1S0W; X-ray; 2.30 A; C/D=37-201.
DR   PDB; 1XXM; X-ray; 1.90 A; C/D=37-201.
DR   PDB; 2B5R; X-ray; 1.65 A; C/D=37-201.
DR   PDB; 2G2U; X-ray; 1.60 A; B=37-201.
DR   PDB; 2G2W; X-ray; 1.80 A; B=37-201.
DR   PDB; 3C4O; X-ray; 1.70 A; B=37-201.
DR   PDB; 3C4P; X-ray; 1.75 A; B=37-201.
DR   PDB; 3C7U; X-ray; 2.20 A; B/D=37-201.
DR   PDB; 3C7V; X-ray; 2.07 A; B/D=37-201.
DR   PDB; 3E2K; X-ray; 2.10 A; C/D=37-201.
DR   PDB; 3E2L; X-ray; 1.87 A; C/D=37-201.
DR   PDB; 3GMU; X-ray; 1.98 A; B=37-201.
DR   PDB; 3N4I; X-ray; 1.56 A; B=37-201.
DR   PDBsum; 1JTG; -.
DR   PDBsum; 1S0W; -.
DR   PDBsum; 1XXM; -.
DR   PDBsum; 2B5R; -.
DR   PDBsum; 2G2U; -.
DR   PDBsum; 2G2W; -.
DR   PDBsum; 3C4O; -.
DR   PDBsum; 3C4P; -.
DR   PDBsum; 3C7U; -.
DR   PDBsum; 3C7V; -.
DR   PDBsum; 3E2K; -.
DR   PDBsum; 3E2L; -.
DR   PDBsum; 3GMU; -.
DR   PDBsum; 3N4I; -.
DR   AlphaFoldDB; P35804; -.
DR   SMR; P35804; -.
DR   IntAct; P35804; 1.
DR   STRING; 443255.SCLAV_4455; -.
DR   GeneID; 61469481; -.
DR   KEGG; sclf:BB341_06630; -.
DR   EvolutionaryTrace; P35804; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033252; P:regulation of beta-lactamase activity; IMP:CACAO.
DR   Gene3D; 3.30.1450.10; -; 2.
DR   InterPro; IPR037873; BamE-like.
DR   InterPro; IPR009099; Beta-lactamas_inhib.
DR   InterPro; IPR024221; BLIP_dom_sf.
DR   Pfam; PF07467; BLIP; 1.
DR   PIRSF; PIRSF009292; B_lactamas_inhib; 1.
DR   SUPFAM; SSF55648; SSF55648; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..36
FT   CHAIN           37..201
FT                   /note="Beta-lactamase inhibitory protein"
FT                   /id="PRO_0000020817"
FT   REPEAT          37..112
FT                   /note="1"
FT   REPEAT          116..201
FT                   /note="2"
FT   DISULFID        66..78
FT   DISULFID        145..167
FT   HELIX           42..47
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   HELIX           54..61
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   HELIX           63..65
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          66..69
FT                   /evidence="ECO:0007829|PDB:3C7U"
FT   HELIX           70..72
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          75..83
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          86..98
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          103..111
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   HELIX           121..126
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   HELIX           133..140
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          143..151
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   TURN            153..156
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          162..168
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   TURN            170..172
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          175..177
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          180..188
FT                   /evidence="ECO:0007829|PDB:3N4I"
FT   STRAND          191..199
FT                   /evidence="ECO:0007829|PDB:3N4I"
SQ   SEQUENCE   201 AA;  20892 MW;  43DD5416631CF0B3 CRC64;
     MRTVGIGAGV RRLGRAVVMA AAVGGLVLGS AGASNAAGVM TGAKFTQIQF GMTRQQVLDI
     AGAENCETGG SFGDSIHCRG HAAGDYYAYA TFGFTSAAAD AKVDSKSQEK LLAPSAPTLT
     LAKFNQVTVG MTRAQVLATV GQGSCTTWSE YYPAYPSTAG VTLSLSCFDV DGYSSTGFYR
     GSAHLWFTDG VLQGKRQWDL V