SYL2_SACS2
ID SYL2_SACS2 Reviewed; 944 AA.
AC O33768; Q9UWZ3;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Leucine--tRNA ligase 2 {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase 2 {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS 2 {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS2 {ECO:0000255|HAMAP-Rule:MF_00049}; Synonyms=leuS;
GN OrderedLocusNames=SSO0589; ORFNames=C21_009;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=10701121; DOI=10.1139/g99-108;
RA Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C.,
RA Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T.,
RA Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E.,
RA Medina N., Peng X., Penny S.L., She Q., St Jean A., van der Oost J.,
RA Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.;
RT "Gene content and organization of a 281-kbp contig from the genome of the
RT extremely thermophilic archaeon, Sulfolobus solfataricus P2.";
RL Genome 43:116-136(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 762-944.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=9209067; DOI=10.1128/jb.179.13.4429-4432.1997;
RA Charlebois R.L., Sensen C.W., Doolittle W.F., Brown J.R.;
RT "Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon
RT Sulfolobus solfataricus P2.";
RL J. Bacteriol. 179:4429-4432(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; Y18930; CAB57710.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK40902.1; -; Genomic_DNA.
DR EMBL; U82227; AAB63016.1; -; Genomic_DNA.
DR PIR; G90205; G90205.
DR RefSeq; WP_009991108.1; NC_002754.1.
DR AlphaFoldDB; O33768; -.
DR SMR; O33768; -.
DR STRING; 273057.SSO0589; -.
DR PRIDE; O33768; -.
DR EnsemblBacteria; AAK40902; AAK40902; SSO0589.
DR GeneID; 44129592; -.
DR KEGG; sso:SSO0589; -.
DR PATRIC; fig|273057.12.peg.598; -.
DR eggNOG; arCOG00809; Archaea.
DR HOGENOM; CLU_004174_0_0_2; -.
DR InParanoid; O33768; -.
DR OMA; AWNMAFQ; -.
DR PhylomeDB; O33768; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IBA:GO_Central.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.90.740.10; -; 1.
DR HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR45794; PTHR45794; 2.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00395; leuS_arch; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..944
FT /note="Leucine--tRNA ligase 2"
FT /id="PRO_0000152145"
FT MOTIF 36..46
FT /note="'HIGH' region"
FT MOTIF 623..627
FT /note="'KMSKS' region"
FT BINDING 626
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 944 AA; 109104 MW; 9ACAFCFBF04160DC CRC64;
MNNIAYKWQT RWEEDKIYES NPNPSKPKFF TTVAFPYPNS PWHIGHGRTY VTGDILARYK
RMRGYNVLFP MAFHYTGTPI MAMADAIAKG DKELIETFKD IYEISPDVIP RMSDPLFMAN
YFKEDIKASM REIGLGIDWR REFTTIDPEF SSFVTWQFHK LQSKGYIVKD THPVGWCPVH
HIPVGMHDTK GDVEPEIGEF VLIYFNSEKG IFPAATLRPE TIFGATALWI NPSEMYVVAS
MLGKKMILSE KAAAKLSFQI DDIEIEEKIK GSKLVGLKVE NPITGKHIAV LGADFVDVSL
GTGVVMSVPA HAPFDYYYSK KTFKNNNIEI IPVITVEGLG NALAKDVVEK NNPKSDEDLK
KLTEYVYRTE YNKGVLRSDL GNLIREEYRN ELKSLGGLPV PKGRELITNF LISKGLGRKI
FEVMNKPVYC RCGTEIVVKI LKDQWFLDYS NKEWKELARK SLSKINVIPE ESRKDFEFTI
EWLEKRACAR TRGLGTPLPW DKKWIIESLS DSTIYMAYYT ISHKIKQYKL SPSKLTQEFW
DYVMLGIGNL EEISEKTGIP SNIIKEFREE FLYWYPLDIR HSGKDLIPNH LTFFIFNHAA
IFQENLWPKA IAVNGLVLYE GKKMSKSLRN IIPLRKGLKM YGVDVMRIAV SSTADMGSDV
NFSESLVKTV GETLRKMYEL FKSLDNYTGD ILGFPEKWLL SRIYEITSST TRHMEALELR
DAVNELLFVF SSDLDEYFGM VSAEGREANN KVLREVLTIW LKLITPFAPH LAEEIWHEIL
KQKTYIVNEG WPEVEGSKMD ELTLLEHEYM KRIVEDIRSI LNIFKGTPKL IKIYALNDSR
YMELLRDAIV ANGQMKKFMD IHKPKSREDA RILQKIFNES LEIDDKMKKL VTNYNINEVD
VLNKLSKYIR RKLNVDILIE PYDEEVKKTY NKEAMPLRPA IIIE
