SYLA_AQUAE
ID SYLA_AQUAE Reviewed; 634 AA.
AC O66680;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Leucine--tRNA ligase subunit alpha;
DE EC=6.1.1.4;
DE AltName: Full=Leucyl-tRNA synthetase subunit alpha;
DE Short=LeuRS;
GN Name=leuS; OrderedLocusNames=aq_351;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC -!- SUBUNIT: Seems to consist of an alpha chain and a beta chain.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; AE000657; AAC06643.1; -; Genomic_DNA.
DR PIR; D70331; D70331.
DR RefSeq; NP_213240.1; NC_000918.1.
DR RefSeq; WP_010880178.1; NC_000918.1.
DR PDB; 3O0A; X-ray; 1.77 A; A/B=225-443.
DR PDB; 3PZ0; X-ray; 2.40 A; A/B/C/D=228-439.
DR PDB; 3PZ5; X-ray; 2.50 A; A/B=228-439.
DR PDBsum; 3O0A; -.
DR PDBsum; 3PZ0; -.
DR PDBsum; 3PZ5; -.
DR AlphaFoldDB; O66680; -.
DR SMR; O66680; -.
DR STRING; 224324.aq_351; -.
DR EnsemblBacteria; AAC06643; AAC06643; aq_351.
DR KEGG; aae:aq_351; -.
DR PATRIC; fig|224324.8.peg.284; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_1_0; -.
DR InParanoid; O66680; -.
DR OMA; DIDWADV; -.
DR OrthoDB; 32262at2; -.
DR BRENDA; 6.1.1.4; 396.
DR EvolutionaryTrace; O66680; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IBA:GO_Central.
DR Gene3D; 3.40.50.620; -; 2.
DR Gene3D; 3.90.740.10; -; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..634
FT /note="Leucine--tRNA ligase subunit alpha"
FT /id="PRO_0000151962"
FT MOTIF 43..51
FT /note="'HIGH' region"
FT STRAND 230..237
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 241..243
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 250..259
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 260..269
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 271..276
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 279..282
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 288..298
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 304..315
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 319..323
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 330..338
FT /evidence="ECO:0007829|PDB:3O0A"
FT TURN 340..342
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 345..351
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 356..358
FT /evidence="ECO:0007829|PDB:3PZ0"
FT STRAND 361..365
FT /evidence="ECO:0007829|PDB:3O0A"
FT TURN 367..369
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 371..380
FT /evidence="ECO:0007829|PDB:3O0A"
FT TURN 396..398
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 411..413
FT /evidence="ECO:0007829|PDB:3O0A"
FT HELIX 418..431
FT /evidence="ECO:0007829|PDB:3O0A"
FT STRAND 434..437
FT /evidence="ECO:0007829|PDB:3O0A"
SQ SEQUENCE 634 AA; 73989 MW; 0119CA3F7A018766 CRC64;
MMKEFNPREI EKKWQKRWEE AGVFKAQEGK PNKFYVLEMF PYPSGRIHMG HVRNYTIGDA
IARYLKMRGK NILHPMGWDA FGLPAENAAI KHGIHPAKWT YENIDYMKKQ LKILGFSYDW
DREIATCDPE YYKWNQWIFL KMLERGIAYR KTAKVNWCPH DQTVLANEQV IEGKCWRCGT
PIVQKEVPSW FLRITAYADR LLEDLKKLEG KWPERVIAQQ RNWIGRSEGA LIRFYVEIEE
PEKFLNCVPE ELKETLLKEK RIYIDVFTTR PDTVFGATFV VLAPEHPLVP VLACIGERLG
NACYSDVENF VEKMKKMSTR ERTMEEDKEG VFLGVYATNP ANGEKIPVWS ANYVLYEYGT
GAIMCVPAHD QRDWEFAKKY DLPIKVVVKP EGAWDFEKGA YEGKGTLVNS DGFDGLDSET
AKRKITEWLQ DRGLGEKKVS YRLRDWNISR QRYWGTPIPV VYCEKCGMVP VPEDQLPVKL
PLDVKFTGQG NPLETSEEFV NTTCPKCGGK ARRETDTMDT FFDSSWYFLR FCDPKNDREP
FSREKVDYWM PVDVYIGGIE HAVLHLLYAR FFQKFLKDLG LVRDDEPFEK LITQGMVLKK
WVSVKKLLDY LGLSEEDEVE ELKKRLEELG ARRA
