ID   SYLC_ENTBH              Reviewed;         860 AA.
AC   B7XHP6;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Probable leucine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.4;
DE   AltName: Full=Leucyl-tRNA synthetase;
DE            Short=LeuRS;
GN   ORFNames=EBI_21702;
OS   Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC   Enterocytozoon.
OX   NCBI_TaxID=481877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA   Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA   Keeling P.J.;
RT   "Patterns of genome evolution among the microsporidian parasites
RT   Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT   bieneusi.";
RL   PLoS ONE 2:E1277-E1277(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA   Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA   Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT   "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT   Enterocytozoon bieneusi.";
RL   PLoS Pathog. 5:E1000261-E1000261(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ABGB01000015; EED44543.1; -; Genomic_DNA.
DR   RefSeq; XP_002649524.1; XM_002649478.1.
DR   AlphaFoldDB; B7XHP6; -.
DR   SMR; B7XHP6; -.
DR   STRING; 481877.B7XHP6; -.
DR   PRIDE; B7XHP6; -.
DR   EnsemblFungi; EED44543; EED44543; EBI_21702.
DR   VEuPathDB; MicrosporidiaDB:EBI_21702; -.
DR   HOGENOM; CLU_004174_0_0_1; -.
DR   InParanoid; B7XHP6; -.
DR   Proteomes; UP000001742; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 3.40.50.620; -; 2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR45794; PTHR45794; 3.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..860
FT                   /note="Probable leucine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388414"
FT   MOTIF           41..51
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           552..556
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         555
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   860 AA;  100606 MW;  2362357DA934B50A CRC64;
     MSENNTKLKY LCDLEEKGRR QCMESEILVD KKKFFITFPY PYMNGKLHLG HLFSISKADI
     FSYYKEMQGF NVLFPFGFHC TGMPISASAK KLKMELANEG EIDISVVNIL KSLGFKLYCS
     GKCTNVECIC QFTDPFHWCR TFPKYCKASL VKYDANIDWR RSFITTDVNP YYDSFVKYQF
     TKLKNCNYIS FGKRYSIFCP VDNQICLDHD RRKGEGVKPI PIVLAILGNN ILVPVQEKDL
     NTIIDNNVEI VTQKEIQWIQ FSINNDVYVA EEDIYNNIKY QVENVYKIKT VTLDGLYPYI
     IYVNSNIRKS KINNKNKILT ENIYNKLIAD RHEKMELIKY DKFIVCYVPE DTVISRSGGK
     CTVALMDQWF LDYSNPEWKE KTKICLNKLT ASKDTKEKLE LALNWINKWG FSRNFGLGTK
     FPYDNSVLID SLSDSTIYMA FYTVKHLLFK DLEGKEPLFP IKYMGYAFWE YVFGNSNEIN
     FIDPNDLNAK KIVEDARSQF NYFYPIDLRV SGKDLINNHL IFFLMNHVAI FKENNWPKRI
     YTNGHLLLNG LKMSKSEGNF LSVDTALERF GTSATRMCLA ICGDTNEDAN FVESMANSFV
     LKLYTLSQNI FLISSNNQSV NVADYTLIDY YLISSIKINE EKTFTSYENI VYRDVIKYGF
     YNNLDTIETY EMFGGSNSNL KHWAYYNMMK QLYPVIPSLA RYFLNEVLTD ISIEFKNIIN
     QQYISGIEYI KELIHKINKF TKHDAKKVNL TLIKHYPPWK ELCMKKIDEL KLIHKNNNDL
     KKIVLKECSQ FIGENNRKKG ILFCMDYLLY KEKYIINFDE ELYTNTFKSI IEQETKKKII
     ILIKDDSTKG EPLCPEINSC