ID   SYLC_NOSCE              Reviewed;         884 AA.
AC   C4V8L1;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Probable leucine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.4;
DE   AltName: Full=Leucyl-tRNA synthetase;
DE            Short=LeuRS;
GN   ORFNames=NCER_100842;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ACOL01000057; EEQ82449.1; -; Genomic_DNA.
DR   RefSeq; XP_002996120.1; XM_002996074.1.
DR   AlphaFoldDB; C4V8L1; -.
DR   SMR; C4V8L1; -.
DR   STRING; 578460.C4V8L1; -.
DR   PRIDE; C4V8L1; -.
DR   EnsemblFungi; EEQ82449; EEQ82449; NCER_100842.
DR   KEGG; nce:NCER_100842; -.
DR   VEuPathDB; MicrosporidiaDB:NCER_100842; -.
DR   HOGENOM; CLU_004174_0_0_1; -.
DR   InParanoid; C4V8L1; -.
DR   OMA; MLIGEFV; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:EnsemblFungi.
DR   GO; GO:1903432; P:regulation of TORC1 signaling; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.620; -; 2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR45794; PTHR45794; 3.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..884
FT                   /note="Probable leucine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388415"
FT   MOTIF           40..50
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           566..570
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         569
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   884 AA;  102520 MW;  909334958DC35622 CRC64;
     MNCNEHESTK LNYLNSLEKS RDVKTDISDK PKYFITFPYP YMNGKLHLGH LYSISKADFM
     SYYKELQGFN VLFPLAFHCT GMPIAALAKK LGEELEGKKT DISTKEIIQN LGFNDVMPFT
     DPVHWIKTFP KLCISSLKTF GANIDWRRSF VTTDINKYYD SFVRWQFFNL KELGYLSFGK
     RHSIYCPLDK QMCLDHDRRK GENIKPVRQI MFKFHLHDKI LLVRQNSIGK PYKLVCGKSL
     EVVSFKYNNT SFLAEKIIFD NLKYQINNVK YKESLVLSMD LKDGLTYDGT NIEVEVIEKN
     ILVVKTDTKS DVELYEKEIK ALNEIEETLF TLSTSSEGIV ESVAKLSVEN TKDLLVQTKH
     FISVYIPEKE VISRSGGICV VSLLDQWYID YSNEKWKSKV KKCLDNLECG PDTRSMLYAG
     IDWVNKWGFS RSFGLGTKIP WDTQYLIDSL SDSTIYMAFY TVKHLLFEDL EGKLEIFPSN
     KLSNDVWDYI FAGKDLVPEL YGYLDLLEKC RNNFQYFYPV DLRVSGKDLI KNHLLFFMFN
     HVALFDEKYW PKRIFTNGHL MLNKEKMSKS TGNFLSVDDA LLKYGKSATR MCLAVCGDTN
     DDANFVEDNA NLFILKIYTF VKEIQKLNEL VRKKSENETI ASYSNADVLL LETVSVNVTE
     ALNAYNSMKY SDVVKFSFFE MVNLINLYNN INGTNILLKN LVYKSATQLL YPIMPDLCRT
     LIEKYFDSEF NLPEPKLKTN KMISAFEYIS ELIKKIAASK QAKNNSCVKI AVGKNYSEWK
     KEIFELIDKI DCPRNNEIKK NKVFIASLLD SVKPILQKSK INITKGNNFV MDYLVNPDKF
     VMKFNEYEII NEFKFYIENM SNKKAILEEH CDAEPLNPII TFSK