ID   SYL_AERHH               Reviewed;         859 AA.
AC   A0KN87;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=AHA_3247;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS   13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC
RC   2358 / NCIMB 9240 / NCTC 8049;
RX   PubMed=16980456; DOI=10.1128/jb.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA   Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA   Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK38227.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000462; ABK38227.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_857738.1; NC_008570.1.
DR   AlphaFoldDB; A0KN87; -.
DR   SMR; A0KN87; -.
DR   STRING; 380703.AHA_3247; -.
DR   EnsemblBacteria; ABK38227; ABK38227; AHA_3247.
DR   KEGG; aha:AHA_3247; -.
DR   PATRIC; fig|380703.7.peg.3242; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..859
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334725"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           619..623
FT                   /note="'KMSKS' region"
FT   BINDING         622
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   859 AA;  96865 MW;  07D0C1F52ABAE7E3 CRC64;
     MQEQYVPQSI EPAVQKHWDA KKTFKAVEKV DKEKFYCLSM FPYPSGRLHM GHVRNYTIGD
     VISRYQRLNG KNVLQPIGWD AFGLPAENAA IKNNTAPAPW TYENIEYMKN QLKMLGLGYD
     WDRELATCKP DYYRWEQWFF TKLYEKGLVY KKTSSVNWCP NDMTVLANEQ VVDNCCWRCD
     TPVEQKEIPQ WFIKITDYAE ELLNDIDNLE GWPEMVKTMQ RNWIGRSEGV NISFAIEGQT
     EHLEVYTTRP DTFMGVTYVG IAAGHPLALQ AAESNPELAA FIEECKNTKV AEAELATMEK
     KGMATGLYAI HPLDGRKVPV WVANFVLMNY GTGAVMAVPG HDLRDHEFAT KYGLEIKPVI
     KPVDGELPNV IDAAYTDKGV LFNSGEFDGL EFQPAFDAIA SKLESLGCGK RTVNFRLRDW
     GVSRQRYWGA PIPMLTLADG TVVPTPEDQL PVLLPEDVVM DGIQSPIKAD AEWAKTTYNG
     QEAFRETDTF DTFMESSWYY ARYCSPDYDK GMLDPAAANH WLPVDQYIGG IEHACMHLLY
     ARFFHKLLRD AGLVNSDEPF KRLLCQGMVL ADAFYYKDEK GGNVWVSPTD VKVERDEKGR
     ITKAVDNEGR EVIHSGMTKM SKSKNNGIDP QLMVERYGAD TVRLFMMFAS PAEMTLEWSD
     SGVEGAQRFL RRLWRITFEH VSAGVAPALN VAALSVDQKA VRRELHKTIA KVSDDVGRRQ
     TFNTAIAAIM ELMNNLAKLG SDEQDRALMQ EALEAVVVML YPITPHIGFE LWKMLGKGDD
     VDHATWPVAD EAAMVETEKL VVVQINGKMR GKLTVPAEIS QADVEKLAMA DASVQKFTDG
     LTVRKVIYVP GKLLNIVAN