SYL_ALKHC
ID SYL_ALKHC Reviewed; 806 AA.
AC Q9K7S8;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=BH3281;
OS Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS / JCM 9153 / C-125) (Bacillus halodurans).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX NCBI_TaxID=272558;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; BA000004; BAB07000.1; -; Genomic_DNA.
DR PIR; A84060; A84060.
DR RefSeq; WP_010899422.1; NC_002570.2.
DR AlphaFoldDB; Q9K7S8; -.
DR SMR; Q9K7S8; -.
DR STRING; 272558.10175904; -.
DR EnsemblBacteria; BAB07000; BAB07000; BAB07000.
DR KEGG; bha:BH3281; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_9; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000001258; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..806
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000151969"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 576..580
FT /note="'KMSKS' region"
FT BINDING 579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 806 AA; 92066 MW; C019DA99B555F479 CRC64;
MSFSHREIES KWQKYWEENK TFKTEEDETR EKFYALDMFP YPSGAGLHVG HPEGYTATDI
LSRMKRMQGY NVLHPMGWDA FGLPAEQYAI DTGNSPAEFT EKNINTFRRQ IKSLGFSYDW
DREVNTTDPD YYKWTQWIFI QLYNKGLAYI DEVAVNWCPA LGTVLANEEV IDGKSERGGH
PVERRPMKQW MLKITEYADR LLEDLEELDW PESIKDMQRN WIGRSEGAEV TFSVDGHDDT
ITVFTTRPDT LFGATYMVLA PEHKLVDAIT TSEQKQAVES YKKEVATKSD LERTELAKEK
TGVFTGAYAI NPVNGEKVPI WIADYVLVSY GTGAIMAVPA HDERDYEFAK TFDLPIKEVV
SGGVIEQEAY TGDGPHVNSE FLNGLSKEEA IEKMIQWLEA EKKGTKKVTY RLRDWLFSRQ
RYWGEPIPVI HWEDGTMSTV PEDELPLELP KMSEIKPSGT GESPLANATD WLEVVDPVTG
KKGRRETNTM PQWAGSCWYY LRYIDPDNER MIADPEKLKK WLPVDIYIGG AEHAVLHLLY
ARFWHKVLYD LGVVPTKEPF QKLYNQGMIL GENNEKMSKS KGNVVNPDDI IDSHGADTLR
LYEMFMGPLD ASIAWSTTGL DGARRFLDRV WRLLVDENTE EKSSKIVNGE GSPELKRAYH
QTVKKVTEDF EELRFNVGIS QLMVYVNEAY KQEELPLDQA EGFVKLLSPV APHLAEELWS
KLGHEGTIAY EPWPTYDEAF LVEDEVEIVV QHNGKVRAKV VVAKDATKEQ MEEAALANER
VKESIDGKTV RKVIVVPGKL VNIVAN
