ID   SYL_ANOFW               Reviewed;         805 AA.
AC   B7GK53;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Aflv_0425;
OS   Anoxybacillus flavithermus (strain DSM 21510 / WK1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus.
OX   NCBI_TaxID=491915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21510 / WK1;
RX   PubMed=19014707; DOI=10.1186/gb-2008-9-11-r161;
RA   Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A.,
RA   Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V.,
RA   Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.;
RT   "Encapsulated in silica: genome, proteome and physiology of the
RT   thermophilic bacterium Anoxybacillus flavithermus WK1.";
RL   Genome Biol. 9:R161.1-R161.16(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000922; ACJ32809.1; -; Genomic_DNA.
DR   RefSeq; WP_012574132.1; NC_011567.1.
DR   AlphaFoldDB; B7GK53; -.
DR   SMR; B7GK53; -.
DR   STRING; 491915.Aflv_0425; -.
DR   PRIDE; B7GK53; -.
DR   EnsemblBacteria; ACJ32809; ACJ32809; Aflv_0425.
DR   KEGG; afl:Aflv_0425; -.
DR   PATRIC; fig|491915.6.peg.435; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_9; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000742; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..805
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000199172"
FT   MOTIF           40..51
FT                   /note="'HIGH' region"
FT   MOTIF           576..580
FT                   /note="'KMSKS' region"
FT   BINDING         579
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   805 AA;  92398 MW;  A208849E5C1DC81C CRC64;
     MSFNHREIEK KWQKYWEENK TFKTTEDDGK RKFYALDMFP YPSGAGLHVG HPEGYTATDI
     LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT EKNINNFRRQ IKSLGFSYDW
     DREVNTTDPN YYKWTQWIFL KLYEKGLAYM DEVPVNWCPA LGTVLANEEV IDGKSERGGH
     PVIRKPMKQW MLRITAYADR LLEDLEELDW PESIKEMQRN WIGRSEGANI HFQVDGHDET
     FTVFTTRPDT LFGATYAVLA PEHPLVEKIT TPEQKEAVEA YLKQIQSKSD LERTDLAKEK
     TGVFTGAYAI NPANGEKLPI WIADYVLMSY GTGAIMAVPA HDERDYEFAK KFNLPIKQVV
     AGGDISKEAY TGDGEHINSD FLNGLNKEEA TKKMIEWLEA NGKGEKKVSY RLRDWLFSRQ
     RYWGEPIPII HWEDGTMTPV PEEELPLVLP KTDEIKPSGT GESPLANIEE WVSVVDPKTG
     KKGRRETNTM PQWAGSCWYY LRYIDPHNDK QLADPEKLEK WLPVDIYIGG AEHAVLHLLY
     ARFWHKFLYD IGVVPTKEPF QKLFNQGMIL GENNEKMSKS KGNVVNPDDI VESHGADTLR
     LYEMFMGPLE ASIAWSTKGL DGARRFLDRV WRLFVEENGE LNPKIVDNPE TDTLERVYHQ
     TVKKVTEDYE ALRFNTAISQ LMVFINEAYK APILPKAYME GFVKLLSPVC PHIAEELWEK
     LGYSNTIAYE AWPAYDEAKL VEDEVEIVIQ VNGKVRAKLH VPADATKEQL EQLAMEDEKI
     KEQIEGKTVR KVIAVPGKLV NIVAN