SYL_AROAE
ID SYL_AROAE Reviewed; 873 AA.
AC Q5P252;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=AZOSEA24870;
GN ORFNames=ebA4386;
OS Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae; Aromatoleum.
OX NCBI_TaxID=76114;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EbN1;
RX PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA Reinhardt R.;
RT "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT bacterium, strain EbN1.";
RL Arch. Microbiol. 183:27-36(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR555306; CAI08612.1; -; Genomic_DNA.
DR RefSeq; WP_011238298.1; NC_006513.1.
DR AlphaFoldDB; Q5P252; -.
DR SMR; Q5P252; -.
DR STRING; 76114.ebA4386; -.
DR PRIDE; Q5P252; -.
DR EnsemblBacteria; CAI08612; CAI08612; ebA4386.
DR KEGG; eba:ebA4386; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_4; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000006552; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..873
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000091289"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 628..632
FT /note="'KMSKS' region"
FT BINDING 631
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 873 AA; 97432 MW; 17317CB2086C0B1F CRC64;
MQDKYTPAAV EATAQQHWES TQAFKVAEDA GKPKYYCLSM FPYPSGKLHM GHVRNYTIGD
VLARYHRMRG FNVLQPMGWD AFGMPAENAA IQNNVPPAKW TYANIDYMKT QLKRLGFALD
WSRELATCKP DYYRWEQWLF TRLYQKGLIY KKLGTVNWDP VDETVLANEQ VIDGRGWRSG
ALIEKREIPM YYMKITAYAD ELLEALDTLT GWPEQVKLMQ KNWIGRSEGV EVHFPYEVST
IGASGVLKVF TTRADTLMGA TYVAVAAEHP LALQAAVNDP ELAAFIEECR HGGVAEADLA
TMEKKGMPTG LRVVHPLTGE HLPVWIANYV LMGYGEGAVM AVPAHDERDF AFATKYRLPI
RMVVRSTRDA YTDTVAPWQD AYAEQGRLVN SGKYDGLHFH DAIEAIAAEL TAKGLGAKRT
QYRLRDWGIS RQRYWGCPIP MIHCADCGDV PVPDEQLPVV LPEDVAVTGR GSPLAKMPRF
YECDCPKCGK PAKRETDTMD TFVESSWYFL RYASADNGQA MVDERVNYWA PVDQYIGGIE
HAILHLLYSR FFTRAMRDEG LVNVSEPFTN LLTQGMVVAE TYYRDADGGK KQWINPADVE
VERDEKGRIV AAKLTADGAP VVIGGIEKMA KSKNNGVDPQ ALVDQYGADT ARLFIIFAAP
PDQQLEWSDS GVEGAYRFLR RVWSFGHAFV SEFRPQLPAD RQLDAVQLPE ALAAVRREIH
VCLRQANYDF GKHQFNTVVS AAMKILNALE KAPRDVAAAH AQVAEEGLDI LLRLLAPITP
HVAHALWQDC GFTGDVLHAS WPEPSEDALR QDEIDLVLQV NGKLRGSLRV AAGASNSAIE
ALALGSETAQ KFMEGKPPRK VVVVPGRLVN IVV
