ID   SYL_AZOC5               Reviewed;         874 AA.
AC   A8IMK0;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=AZC_0567;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC   13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; AP009384; BAF86565.1; -; Genomic_DNA.
DR   RefSeq; WP_012169098.1; NC_009937.1.
DR   AlphaFoldDB; A8IMK0; -.
DR   SMR; A8IMK0; -.
DR   STRING; 438753.AZC_0567; -.
DR   EnsemblBacteria; BAF86565; BAF86565; AZC_0567.
DR   KEGG; azc:AZC_0567; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_5; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..874
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000071108"
FT   REGION          614..634
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           627..631
FT                   /note="'KMSKS' region"
FT   BINDING         630
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   874 AA;  97619 MW;  B7DC946BFD5677C8 CRC64;
     MSTDRYNARD AEPRWQAIWN ERGIFRTRND DPRPKFFVME MFPYPSGRIH IGHGRNYVMG
     DVLARYKRMQ GFNVLHPMGW DSFGLPAENA AIERGIHPKS WTYENIASMK EQLQLLGLSL
     DWNREVATCD PSYYAEQQRL FLDLFENDLA YRKESEVNWD PVDNTVLANE QVIDGRGWRS
     GAVVERRKLN QWFFRITAFA QELLDAIDTL DRWPDKVRLM QRNWIGRSEG LEVLFELSKG
     SVAAAGTSAV KVYTTRPDTL FGASFLALSP DHPLTQHLAA TRPDLAAFVA DCKTGGTSAE
     AIETQEKKGF DTGLTVVHPL DAARTVPVYV ANFVLMDYGT GAIFGCPAHD QRDLDFARKY
     GLTVTPVVLP PGADPAVFAV GSEAYDGEGT LYNSQFLDGL SIADAKEQVA RRLERFRVGE
     QPQGTRKVNF RLRDWGISRQ RYWGCPIPII HCDSCGPVAV PREQLPVELP DDVTFDRPGN
     PLDRAKAWRD VPCPKCGAPA RRETDTMDTF VDSSWYFLRY ASDNPDKPLD KDAVRHWLPV
     DQYIGGIEHA ILHLLYSRFF TRALKACGRV DVAEPFAGLF TQGMIVHETY KDKDGRWLFP
     EEVKLLGDGK AEKLDDGSPV TVGPPEKMSK SKKNVVPPEV VVDTYGVDAG RWFMLSDTPP
     ERDSEWTQSG IEGAWRFVQR VWRQVQEAIE LGAPKGADKP ASFDAAATAL RRAAHGLAHQ
     VADDVERLRF NVAVAHVHEF ANAFGQAIAS ARTASPVPAD LAFALREAAE IVTRVVGPMV
     PHLAEECWAA LGYDTLLAEA AWPKAEPELL VENTITLPIQ INGKKRDDIT VPRDATAAEV
     EAAVLEMESV RRALDGKAPK RIIVVPQRIV NVVA